不同铜浓度的猪粪对玉米叶片蛋白质和氨基酸含量的影响.pdfVIP

第33卷第6期 湖南农业大学学报(自然科学版) Vo1．33 No．6 2007年 12月 Journal of Hunan Agricultural University(Natural Sciences) Dec．2007 文章编号：1007．1032(2007)06．0760—05 不同铜浓度的猪粪对玉米叶片蛋白质和氨基酸含量的影响 高凤仙，常嵩华，田科雄，钟元春，陈宇光 (湖南农业大学 动物科学技术学院，湖南 长沙 410128) 摘 要：采用盆栽试验研究了不同铜浓度的猪粪对玉米叶片蛋白质、氨基酸含量的影响．供试猪粪为猪采食基 础Et粮添加铜250 mg／kg排出的粪，设每盆施猪粪100，200，300 g 3个试验组，对照组猪粪为猪采食基础Et粮排 出的粪，每盆施300 g．移栽3叶期玉米后 10，20，30，40 d测定玉米叶片可溶性蛋白质含量、游离氨基酸总量 和脯氨酸含量．结果表明，玉米移栽后 10—20 d，叶片可溶性蛋白质、游离氨基酸总量显著高于对照组；玉米移 栽后30~40 d，随着施用猪粪含铜浓度的增大和时间的延长，叶片可溶性蛋白质含量、游离氨基酸总量显著低于 对照组，铜浓度越高，其含量下降越快；试验全期，随猪粪铜浓度的增加玉米叶片脯氨酸含量显著增加． 关 键 词：猪粪；铜；可溶性蛋白质；游离氨基酸；脯氨酸；玉米 中图分类号：$141．2 文献标识码：A Effects of copper in pig feces on protein content in leaves of maize GAO Feng-xian，CHANG Song-hua，TIAN Ke-xiong，ZHONG Yuan-chun，CHEN Yu-guang (College ofAnimal Science and Technology，HNAU，Changsha 410128，China) Abstract：A pot experiment was conducted to study the effects of different levels of copper in pig feces on protein content in leaves of maize．Pig feces in 3 treatment groups came from those pigs fed with basal dietary supplemented 250 mg／kg Cu，and three different amounts：100，200，300 g／pot were added into each treatment respectively．Pig feces ofthe controlling group were from those only fed with basal dietary，and 300 g was used in each pot．Contents of soluble protein，totalfree amino acidandprolineinleavesweretested after10，20，30，40 days ofthetransplantofmaize．The results showed that contents of soluble protein and total free amino acid in leaves were higher tO controlling group from 10—20 d after planting．With time passing by．soluble protein content and total free amino acid content in leaves were obviously lower than those of the controlling group between 20 d