微生物分子生态学技术及其在石油污染土壤修复中的应用现状与展望_吴作军.pdfVIP

DOI:10.16085/j.issn.1000-6613.2010.05.023 化 工 进 展 2010 年第29 卷第5 期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ·789 · 特约评述 微生物分子生态学技术及其在石油污染土壤修复中的 应用现状与展望 吴作军，卢滇楠，张敏莲，刘 铮 （清华大学化学工程系，北京 100084 ） 摘 要：概述了微生物分子生态学技术的原理及在土壤生物修复中的应用。该技术可以提供土壤微生物群落多 样性信息，跟踪降解菌株（群）的定植及发挥作用的过程，展现投加菌株与土著菌群相互作用的信息，为生物 修复中降解菌的筛选、修复过程的强化及修复结果的评估提供生物学信息。展望了该技术在土壤生物修复中应 用的前景。 关键词：土壤生物修复；分子生态学；微生物分子生态学；分子生物学 中图分类号：TQ 033 文献标识码：A 文章编号：1000-6613 （2010 ）05-0789-07 Progress in applications of microbiological molecular ecology in bioremediation of petroleum contaminated soil WU Zuojun，LU Diannan ，ZHANG Minlian ，LIU Zheng （Department of Chemical Engineering ，Tsinghua University ，Beijing 100084 ，China ） Abstract ：This review starts with a brief introduction on principles and methods of microbiological molecular ecology as well as their applications in soil remediation. In addition to the genetic information of soil microbial community ，the use of microbial molecular ecological tools enables the monitoring of the growth and function of inoculant degrading microbials ，as well as their interaction with the indigenous microorganisms. These information are of fundamental importance for the selection and screening of degradation microbial communities ，the intensification of degradation process ，and the evaluation of remediated soil. Application perspective of microbiological molecular ecology is also discussed. Key words：soil biorem