槐花总黄酮对大鼠糖尿病模型血清胰岛素、瘦素和C-肽水平.PDFVIP

槐花总黄酮对大鼠糖尿病模型血清胰岛素、瘦素和C-肽水平的影响 苗明三1 2 1 ，李彩荣 ，陈元朋 (1.河南中医学院，郑州 450008 ；2.河南省中药发展工程技术研究中心，郑州 450001) 摘要：目的 探讨槐花总黄酮对链脲佐菌素性大鼠糖尿病模型血清胰岛素、瘦素和C-肽水平的影响。方法 以舌下静脉 注射链脲佐菌素 60 mg·kg-1 制备大鼠糖尿病模型，随机分为 5 组：槐花总黄酮高(600 mg·kg1) 、中(300 mg·kg1) 、低 (150 mg·kg1)剂量组、二甲双胍组(208 mg·kg1)和模型组(给予同体积生理盐水) 。另取健康大鼠设为空白对照组，给予同 体积生理盐水。每天给药 1 次，连续给药30 d ，于第10，20 ，30 天分别测空腹血糖值，第30 天测血清胰岛素、胰岛素 抗体、瘦素和C-肽水平。结果 模型组从实验开始至第30 天血糖水平显著升高，第30 天血清瘦素水平显著升高，血清 胰岛素水平及C-肽水平显著降低，说明链脲佐菌素大鼠糖尿病模型制备成功。与模型组比，槐花总黄酮各剂量组第20 ， 30 天可显著降低模型大鼠血糖水平，第30 天可显著升高血清胰岛素水平和C-肽水平，显著降低血清瘦素水平。结论 槐 花总黄酮对链脲佐菌素性大鼠糖尿病模型有良好的治疗作用，可使血清葡萄糖及瘦素水平显著降低，血清胰岛素水平和 C-肽水平显著升高。 关键词：槐花总黄酮；糖尿病模型；胰岛素抗体；瘦素；C-肽 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2011)10-0896-03 Effects of Total Flavonoids of Sophorae Flos on the Levels of Insulin, Leptin and C-Peptide in Serum in Diabete Rat Model 1 2 1 MIAO Mingsan , LI Cairong , CHEN Yuanpeng (1.Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China; 2.Henan Engineering and Research Center for TCM, Zhengzhou 450001, China) ABSTRACT: OBJECTIVE To investigate the effect of total flavonoids of Sophorae Flos (TFSF) on the levels of insulin in serum, leptin and C-peptide in diabete rats which was established by streptozotocin. METHODS The diabete rat model was established by injected with 60 mg·kg1 streptozotocin, and then randomized into 5 groups: large dosage TFSF group(600 mg·kg1), middle dosage TFSF group(300 mg·kg1), small dosage TFSF group(150 mg·kg1), metformin group(208 mg·kg1), and model group. Control group was set up with healthy normal rats. All the rats were administrated once a day for 30 days. Blood glucose in the limosis were measured on the 20, 30 day and then rats were sacrificed. The levels of insulin, insulin antibody, leptin, C-peptide in orbital blood were measured. RESULTS Compared with