高效液相色谱荧光法测定土壤中16种多环芳烃.pdfVIP

2010 ，19（2） 福建分析测试 Fujian Analysis Testing 69 高效液相色谱荧光法测定土壤中16种多环芳烃 罗世榕 (福州市高新技术产业创业服务中心，福建 福州 350002) 摘 要：本文介绍了采用高效液相色谱、荧光检测器测定土壤中16种多环芳烃。通过对液相色谱柱的选择、色谱条 件、荧光激发和发射波长等条件的优化，实现16种多环芳烃完全分离。特别是对16种多环芳烃的激发和发射波长进 行选择，进一步提高检测灵敏度、选择性，降低了干扰。在优化的实验条件下，荧光检测器的检出限为0.09～ 1.57ng/mL，样品加标回收率为86%- 108%。 关键词：高效液相色谱；荧光检测；多环芳烃；土壤 中图分类号：O657.72 文献标识码：A 文章编号：1009- 8143(2010)02- 0069- 04 Determination of 16 Polycyclic Aromatic Hydrocarbons in Soil by HPLC with Fluorescence Detection Luo Shi-rong （Fuzhou High- Tech Innovation Service Center, Fuzhou, Fujian350002,China） Abstract ：High performance liquid chromatographic with fluorescence detection method was applied the determination of 16 polycyclic aromatic hydrocarbons (PAHs)in soil.The method realized the complete separation of 16 PAHs and high sensitive fluorescence determination after optimizing analysis conditions,including liquid chromatographic conditions, excitation and emission wavelength.The accuracy for the determination may be improved with fluorescence detection.Especially for 16 PAHs in excitation and emission wavelengths to make your selection, further improved the detection sensitivity, selective,and reduced interference.Under optimizing conditions,The detection limits of the fluorescence detection was 0.09 ～1.57 ng ／mL,and the recoveries of standard addition were 86%- 108%. Keywords ：High performance liquid chromatography ; fluorescence detection;polycyclic aromatic hydrocarbons;soil. 土壤中多环芳烃(PAHs)主要来源于植物枝干、 化学性质相近，沸点较高，土壤中PAHs 为微量存 塑料等石化产品的焚烧过程中不完全燃烧生成，并 在，样品组成复杂、受干扰严重。因此准确测定 被吸附到飘尘上以气体的形式存在大气层中，通过