低温海水浸泡协同力竭性游泳致应激性溃疡的大鼠模型建立.pdfVIP

《东南国防医药92007年12月第9卷第6期 ● 论 著 ● 低温海水浸泡协同力竭性游泳致应激性溃疡 的大鼠模型建立 骆益宙 ，缪冬梅 ，傅继华。 (1．解放军第454医院肿瘤科，江苏南京 210002；2．南京港口医院内科，江苏南京 210011；3．中国药科大学生理教研室，江苏南京 210009) [摘 要] 目的 通过低温海水浸泡、力竭性游泳刺激，建立应激性溃疡的动物模型。方法 Wistar雄性大鼠56只分4组，A、B、c 组各16只，D组8只。A组0 C海水浸泡同时给予疲劳性游泳刺激；B组单纯0 C海水浸泡；C组力竭性游泳刺激；D组为正常对照组。 通过观察胃粘膜溃疡的发生率和Guth法评价溃疡分数。溃疡局部胃组织进行病理学检查。结果 A组发生应激性溃疡12只 (75．0 )，B组发生应激性溃疡7只(43．8 )，C组发生应激性溃疡6只(37．5 )，D组未发现溃疡。A组应激性溃疡发生率高于B、 c组(Po．O1)，B、C组之间无显著性差异(Po．05)。溃疡分数A组(33．5±1．2)、B组(23．1±11．6)、C组(27．7土12．2)、D组(1．0 ±0．2)，A组和B、c组之间存在显著性差异(JPo．01)，B、C组之间无显著性差异(JPo．05)。结论 低温海水浸泡加力竭性游泳 刺激可有效诱导应激性溃疡，是理想的动物模型。 [关键词] 应激；溃疡病；动物模型 中图分类号： R573．1 文献标识码： A 文章编号： 1672—271X(2007)06—0420—02 Establishment of rat stress ulcer model induced by cold sea water—immersion and exhausted swimming LUO Yi—zhou ，MIAO Dong—mei ，FU Ji—hua。 (1．Oncology Department of the 454 th Hospital of PLA，Nanjing 210002，Jiangsu， China；2．Intenal Department of Port Hospital，Na， ng 210011，Jiangsu，China；3．Department of Physiololgy，China Pharmaceuti— cal University，Nanjing 210009，Jiangsu，China) [Abstract] Objective To establish a stress ulcer model induced by cold sea water—immersion and exhausted swimming．Methods 56 male Wister rats were divided into 4 groups，16 rats was included in A，B and C group，the other 8 rats in group D．Rats in group A were immersed in 0 C cold sea water and exhausted by swimming，in group B were immersed in 0 C cold sea water，in group C were exhausted by swimming，group D as contro1．The rate of gastric ulcer in experimental rats and ulcer index by Guth method were calculated．The ulcer 1esion was collected for further pathological examination．Results Stress ulcers occurred in group A(75．O’ )，B(43．8 )and C(37．5 )，no stress ulcer was obversed in group D．The occurrence rate of stress ulcer in group A was higher than group B and C (JPO．01)，no difference was found between group B and C(JPO．05)．Ulcer index in group A (33．5土1·2)was higher than group B(23．1土11．6)a