HPLC法测定山绿茶中芦丁的含量-李霞-江西中医学院.doc

江西中医学院 2008届毕业论文 论文题目：HPLC法测定山绿茶中芦丁的含量 课题来源：自选课题 论文性质：实际应用 论文发表： —— 学生姓名：李 霞 学　　号：2004107036 班　　级：04中制 导师姓名：李建勋　　　　职　　称：药师 导师签名： 实习单位：同溢堂药业有限公司 单位地址：福建省漳州市漳浦县绥安工业区 完成时间：２００８年５月  目　　　　录 一、论文 １、标题和作者···························································3 ２、中文摘要及关键词··················································3 ３、外文摘要及关键词··················································3 ４、前言····································································4 ５、正文····································································4 ６、讨论或小结···························································9 ７、参考文献······························································9 ８、综述（必要时）······················································10 ９、致谢及声明···························································11 二、论文简介 HPLC法测定山绿茶中芦丁的含量 李霞（江西中医学院330006） 李建勋（同溢堂药业有限公司363200） 摘要 目的： 建立山绿茶中芦丁的含量测定方法。方法: 采用ODS C18柱，5μm，4.6×250mm；乙腈-2.5％醋酸溶液（14.2∶85.8）为流动相；流动速为1.0ml/min； 柱温为30℃；检测波长为254nm。结果:芦丁在0.01833mg/ml～0.1833mg/ml范围内与峰面积呈良好的线性关系（r=0.9998)。平均回收率为97.5%(RSD=1.07%,n=6）。结论:该方法简便、准确、重现性好。可用于为山绿茶中芦丁含量测定。 关键词 山绿茶；芦丁； 高效液相色谱法 DETERMINATION THE CONTENT OF RUTIN IN SHANLUCHA BY HPLC ABSTRACT Purpose：To establish a method for determination of rutin in shanglucha. Method: HPLC analysis was carried out using ODS C18 column（4.60×250mm 5um） and acetonitrile-0.1% acetic acid solution(14.2:85.8) as the mobile phase；flow rate 1.0ml/min and column temperature at 30℃；The detection wavelength was 254 nm. Results: The range of linear relationship was 0.01833mg/ml～0.1833mg/ml (r=0.9998). The average recovery was 97.5%( RSD=1.07%,n=6). Conclusion:The method is simple,accurate,reproducible and suitable for determinating the rutin in shanglucha. Key words Shanglucha；rutin；High Performance Liquid Chromatographic(HPLC) 前言 山绿茶为冬青科植物海南冬青Ilex hainanensis Merr.的干燥叶，主要分布于广西、广东一带。山绿茶为广西少数民族民间中草药，使用历史悠久，已收载于《广西中药材标准》(1990年版)[1]，具有清热解毒，消肿止痛，活血通脉的功效，主要用于降血压、降血脂、降胆固醇及冠心病、脑血管意外所致的偏瘫，以及风热感冒、肺热咳嗽、咽喉水肿、扁桃体炎、痢疾