CRISPRCas系统介导的水产生物基因组编辑研究探索.pdfVIP

水产学杂志 第 28 卷第6 期 Vol. 28 ,No.6 2015 ~ 12 月 CHINESE JOURNAL OF FISHERIES Dec.2015 文章编号:1005-3832(2015)06-0038-06 CRISPR/Cas 系统介导的水产生物基因组编辑 研究探索 柳项 12 ，李尚琪 2 ，徐鹏 2 (1.山西农业大学动物科技学院，山西晋中 030801 ; 2.中国水产科学研究院生物技术研究中心，北京 l ∞141 ) 摘要:基因组编辑技术是研究基因功能的重要方法，特异性人工酶ZFN (Zinc- finger nucleases) 、 TALEN(Tran­ scription activator like effector nucleases)和 CRISPRlCas 系统则使基因编辑变得更容易。本文通过介绍CRISPRlCas (clustered re伊larly inter百paced short palindromic repeats/CRISPR -associated system) 系统的基本结构及作用原理， 剖析潜在的问题，综述其在水产生物基因组编辑中的应用研究进展，以期为这一领域的研究提供参考。 关键词:CRISPRlCas 系统;基因组编辑;水产生物 申图分类号:S917 文献标志码:A Exploration of Aquatic Organism Genome Editing Mediated by CRISPRlCas System l L阳Xiang ，2， LI Shang-qF, XV Pen矿 ( 1. College ofAnimal Science and Technology, Shanxi Agricultural University,Jinzhong 030801 , China; 2. Centre for Applied Aquatic Genomics, Chinese Academy ofFishery Sciences, Beijing 100141, China) Abstract: In modern biological research , genome editing technology is one of the key factors to realize gene function , Sequence-spe- cific nucleases like ZFNs (Zinc-finger nucleases) , TALENs (Transcription activator like e能ctor nucleases) and the CRISPRlCas9 system (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 sys旬m) have greatly expanded the genome editing possibilities in model organisms. The basic s住ucωre and principle of the CRISPRlCas9 system are described, and potential challenges of the CRISPRlCas system are discussed based on related l