_大鼠Langendorff离体心脏灌流模型的制备经验及其影响因素分析.pdfVIP

184 中药药理与临床20 14 ;30 (6) Experimental study on the model of sub-healthy rats caused by long-term irregular life schedule and disorder diet Zhang Yueli1 ，Lv Guiyuan2 ，Ding Ting1 ，Deng Mengjiao1 ，Lin Weiyi1 ，Chen Suhong1 ＊＊ 1 (Wenzhou Medical University ，Zhejiang Wenzhou 325035 ; 2 Zhejiang Chinese 8Medical University ，Zhejiang Hangzhou 310053) Objective :To simulate modern people irregular life schedule and disordered diet resulting in disrupted biological clock ，explore and evaluate the model of the sub-healthy rats caused by irregular life style． Methods :Use long-term 24h / 24h interval sleep deprivation com- bined with diet disorder method to break the normal life rhythm in rats． The detailed method is as follows :On odd-numbered days ，model rats live a 24h normal life and on a high-sugar plus high fat diet (overeating sugar and fat)，on even-numbered days use 24h sleep deprivation and feed model rats Chinese cabbage (only vegetarian food)． Then compare the differences between the normal group and the model group in the areas of hematology ，behavioristics ，electrocardiology ，and observe the reversibility of the model． Ｒesults :Compared with normal rats ， ①In behavioristics area :After modeling 2 weeks ，model rats appeared flushed ears phenomenon ，rats＇holding power significantly reduced (P ＜ 0 ． 01);After modeling 4 weeks ，model rats＇ pain threshold significantly reduced (P ＜ 0 ． 01);After modeling 5 weeks ，model rats＇ facial temperature significantly rised (P ＜ 0 ． 01);Stop modeling one week ，model rats＇facial temperature went back to normal ，while holding power and pain threshold didn＇t obviously recover． ②In blood routine test :After modeling 5 week