和16SrDNAPCR-RFLP研究.PDFVIP

微生物学通报 MAY 20, 2008, 35(5): 754~759 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 分离自补骨脂等宿主根瘤的 24 株菌的数值分类 和 16S rDNA PCR-RFLP 研究 郝云婕 王孟兆 刘 磊 韩素贞* ( 100037) 摘 要: 采用数值分类和 16S rDNA PCR-RFLP 对分离自云南省豆科植物补骨脂(Psoralea coryli- folia ) 、葛藤(Pueraria lobata) 、杭子梢(Campylotropis macrocarpa)等宿主的24 株菌及 10 株根瘤菌 参比菌株进行了研究。数值分类结果表明, 在 84%相似性水平上, 所有的菌株可分为 3 群：群Ⅲ 为未知菌群, 群Ⅰ为慢生菌群, 群Ⅱ为快生和中慢生菌群。从依据 16S rDNA PCR-RFLP 分析建立 的树状图来看, 在 70%相似性水平上, 所有的菌株可分为 5 个系统发育分支：分支Ⅰ和Ⅴ没有参 比菌株, 为未知分支; 分支Ⅱ为 Agrobacterium-Sinorhizobium-Rhizobium , 分支Ⅲ为 Mesorhizo- bium, 分支Ⅳ为Bradyrhizobium 。数值分类和16S rDNA PCR-RFLP 的结果部分一致, 有2 株菌与 A. tumefaciens IAM13129T 聚在一起。 关键词: 根瘤菌, 数值分类, 16S rDNA PCR-RFLP Numerical Taxonomy and 16S rDNA PCR-RFLP of Rhizobial Strains Isolated from Psoralea corylifolia etc * HAO Yun-Jie WANG Meng-Zhao LIU Lei HAN Su-Zhen (Life Science College , Capital Normal University, Beijing 100037) Abstract: 24 strains obtained from root nodules of Psoralea corylifolia , Pueraria lobata, and Campylotropis macrocarpa of Yunnan province were studied with numerical taxonomy and 16S rDNA PCR-RFLP. Results of numerical taxonomy indicated that all strains included 10 reference strains were divided into 3 groups at 84% similarity. Group III is an unknown group with no reference strains. Group I is slow-growing kind, and group II fast and middle-slow