益气活血复方对自发性高血压大鼠左室心肌形态结构的影响论文.docVIP

　　益气活血复方对自发性高血压大鼠左室心肌形态结构的影响论文 张纯， 陈利国，戴勇， 胡小勤， 郑佩娥 【关键词】 益气活血复方；,,自发性高血压大鼠；,,,左室肥厚；,,形态结构 摘要：目的探讨益气活血复方对自发性高血压大鼠（spontaneously hypertensive rats, SHR）左室肥厚(left ventricular hypertrophy, LVH)及左室心肌形态结构的影响。方法雄性14周龄SHR 50只，随机分为复方高剂量组（SHRY1）、复方中剂量组（SHRY2）、复方低剂量组（SHRY3）、模型对照组（SHRC）、硝苯地平组（SHRX）。监测尾动脉收缩压（systolic blood pressure, SBP），每4周1次，8周后取材。测算心脏重量指数（heart ass index, LVMI）；光镜、透射电镜观察左室心肌细胞形态结构的改变。结果（1）与SHRC组比较.freelorphological structure of myocardial cells in left ventricle of spontaneously hypertensive rats(SHR). MethodsFifty fourteenale SHR ly into 5 groups: SHRY1(treated oderate dose of Yiqihuoxue decoction), SHRY3(treated easured before and during treatment every four ent, all rats ass index(LVMI) orphological and structural changes of myocardial cells in left ventricle of SHRs icroscope and transmission electron microscope(TEM). Results(1) After 4 or 8 ent, pared each treatment group ent, pared SHRY1, SHRY2 and SHRX icroscope uscle fibers ent, as e hypertrophic nuclear or pyknosis of myocardial cells. The results under TEM e left ventricular tissues obtained from SHRC group, nuclear membrane shrank and nuclear chromatin condensed and congregated under nuclear membrane like speckle, abutting against the nuclear envelope; and pyknosis e myocardial cells arkedly se disorganized or disrupted mitochondrial cristae entioned pathological changes of SHRY1 eliorated slightly in SHRY2 and SHRX; but there provement in SHRY3. ConclusionYiqihuoxue decoction and nifedipine can reduce the SBP, HI and sloages of left ventricle in SHRs during the process of LVH. Key l药液含2 g生药）。硝苯地平，10 mg/片（广东华南制药厂生产，批号：040602）。 1.2 动物及分组 清洁级雄性14周龄SHR大鼠50只，体重(257±12) g，购自上海斯莱克实验动物有限责任公司。普通饲料喂养，避免噪音及其他干扰，观察期为8周。SHR随机分为5组，即复方高剂量组（SHRY1）、复方中剂量组（SHRY2）、复方低剂量组（SHRY3）、模型对照组（SHRC）、硝苯地平组（SHRX），每组10只。按体表面积法［1］换算：大鼠复方每日用量为13 g/kg体重，硝苯地平每日用量为5.4 mg/kg体重。以复方换算剂量为SHRY2组给药剂量，SHRY1、SHRY3组分别为其3、1/3倍；SHRX组按大鼠换算剂量灌胃。即：SHRY1、SHRY2、SHRY3、SHRX组分别予以39 ，13，4.3 ，5.4 mg/kg d药物灌胃；SHRC组予等体积蒸馏水灌胃。动物禁食12 h后灌胃，1次/d。将药物按不同剂量