神经营养因子家族及其受体在重组人骨形态发生蛋白论文.docVIP

　　神经营养因子家族及其受体在重组人骨形态发生蛋白论文 【关键词】 骨形态发生蛋白 摘 要：［目的］通过观察重组人骨形态发生蛋白2（rhBMP2）诱导成骨过程中神经营养因子（NTFs）家族及受体的表达，探讨神经营养因子在BMP骨诱导中的作用。［方法］建立小鼠右侧股后部异位成骨模型，实验组植入rhBMP2胶原复合物，对照组仅植入相同体积的胶原海绵。分别于术后7、14和21 d取材，进行组织学、免疫组化及RTPCR检测。［结果］组织学证实rhBMP2胶原复合物具有良好的骨诱导能力，而对照组未见成骨。术后第7 d，在大量软骨形成期NGF和TrkA阳性染色达到高峰，成纤维细胞、成软骨细胞、软骨细胞、肥大软骨细胞和成骨细胞中均有阳性表达；BDNF在软骨细胞及软骨基质中有阳性染色.freelRNA于术后第7 d表达最高，与免疫组化中蛋白质的表达结果相一致。［结论］NTFs及其受体在rhBMP2诱导成骨过程明显表达，提示NTFs可能通过直接和间接的方式协同BMP的诱导成骨过程。 关键词：骨形态发生蛋白； 骨诱导； 神经营养因子； 受体 Abstract：［Objective］To identify the expression of neurotrophic factors（NTFs）and their high affinity receptors（Trk）during the bone induction by rebined human bone morphogeic protein2（rhBMP2）and discuss possible roles of NTFs on the osteoinductive activity of BMP［Method］After a total of 36 ICR mice ental group and control group at random,rhBMP2/collagen sponge and collagen sponge planted into the right thigh muscle pouches of tplanted sites of toved on the 7th,14th and 21st day after implantationHistological,immunohistochemical and RTPCR analyses ed to detect osteoinductive effect of rhBMP2 and the expression of NTFs and Trk［Result］RhBMP2/collagen sponge displayed a potent ability inducing bone formation,ental group,NGF and TrkA positive immunostaining reached the peak in the stage of chondrogenesis,including chondroblasts,chondrocytes,hypertrophic chondrocytes and osteoblasts,then decreased in the number of positive cells and the intensity of immunostaining on the 14th and 21st dayBDNF atrix,and TrkB plantationExpression of NT3 ilar to that of NGF,RNA of NTFs peaked at 7 days after the implantation,then decreased,.freelmunohistochemical results［Conclusion］Many different kinds of cells are induced by rhBMP2 express NTFs and Trk,ay play an important role in the osteogenesis initiated by BMP through direct and indirect pathorphogeic protein; Osteoinduction; Neurotrophic factors; Receptor 骨形态发生蛋白（bone morphogeic protein，BMP）能诱导未分化间充质细胞不可逆地分化形成骨和软骨，在骨折愈合和骨修复过程中发挥着重要作用。研究表明，在骨折愈合中，除了BMP的诱导成骨作用外，尚有其他众多因子的表达和参与，