在体微透析观察大鼠急性一氧化碳中毒后纹状体多巴胺及其代谢产物变化.docVIP

在体微透析观察大鼠急性一氧化碳中毒后纹状体多巴胺及其代谢产物变化摘要： ［目的］ 研究急性一氧化碳（carbon monoxide，CO）中毒对大鼠脑纹状体多巴胺（DA）及其代谢产物二羟苯乙酸（DOPAC）、高香草酸（HVA）水平的影响。 ［方法］ 将大鼠随机分成对照组和CO中毒组，采用腹腔注射CO法染毒，首次染毒剂量为120 ml/kg，然后每隔4 h注射一次，共3次维持剂量60 ml/kg，对照组以同样方法和剂量注射空气。观察首次染毒后24 h 内各组动物血碳氧血红蛋白（HbCO）浓度变化，同时利用在体微透析技术观察脑纹状体DA及其代谢产物在末次染毒后11 h内及第10 d的变化特点。 ［结果］ 腹腔注射CO染毒后，大鼠血HbCO浓度迅速增高，在首次染毒后1～2 h达高峰，维持剂量连续染毒时，血HbCO浓度可维持在55%以上达15 h，末次染毒6 h后降至正常水平；末次染毒后，大鼠脑纹状体DA水平增加，其代谢产物明显减少，至第4～6 h开始逐渐恢复，第11 h恢复到正常水平；末次染毒后第10天中毒组动物纹状体DA水平与对照组比较再次明显降低，其代谢产物水平明显升高。 ［结论］ CO中毒血HbCO浓度恢复正常水平后，提示DA及其代谢产物再次出现异常变化可能与急性CO中毒迟发性锥体外系神经精神症状有关 关键词：一氧化碳；急性中毒；多巴胺；纹状体；锥体外系症状 Characterization of Dopamine and Its Metabolites in the Striatum of Rats Due to Carbon Monoxide Poisoning as Determined by in vivo MicrodialysisGUAN Li，ZHAO Jin-yuan*，WEN Tao，ZHANG Yan-lin（Research Center of Occupational Medicine，The Third Hospital of Peking University，Beijing 100083，China） Abstract：［Objective］ To explore the effect of Carbon monoxide（CO）poisoning on the changes of dopamine（DA）and its metabolites dihydroxyphenylacetic acid（DOPAC）and homovanillic acid（HVA）in the striatum of free-moving rats. ［Method］ Rats were divided at random into two groups，control group and CO intoxicated group. CO was injected into rats of the intoxicated group intraperitoneally at a dosage of 120 ml/kg for the first time，and then repeated injections with CO（60 ml/kg）for 3 times at 4 h intervals was provided.Same amount of air was injected into the control rats intraperitoneally at the same intervals.HbCO levels in the blood of rats was measured for 24 hours after the first CO administration，and changes of DA and its metabolites levels in the striatum was observed for 11 hours and at the 10th day after the last CO administration，as determined by means of brain microdialysis. ［Results］ Compared to control group，HbCO level increased much higher and reached the peak at 1 to 2 hours after the first CO administration，and it could be kept above 55% for 15 h. CO poisoning significantly changed extrac