氡及其子体诱发大鼠体细胞HPRT基因位点突变.docVIP

氡及其子体诱发大鼠体细胞HPRT基因位点突变摘要： ［目的］ 用多核细胞法研究氡及其子体诱发大鼠外周血淋巴细胞和气管-支气管上皮细胞的次黄嘌呤鸟嘌呤磷酸核糖转移酶（HPRT）基因位点突变情况。 ［方法］ Wistar雄性大鼠24只，采用HD-3型多功能移动式氡室进行动态吸入染毒，按照氡染毒的累计暴露量，分为3个实验组和1个对照组；获取大鼠外周血淋巴细胞和气管-支气管上皮细胞，以多核细胞法检测该两种细胞的HPRT基因突变频率。 ［结果］ 大鼠外周血淋巴细胞和气管-支气管上皮细胞的HPRT基因突变频率均随氡累积暴露剂量的增加而相应增加，剂量-效应关系明显，拟合的函数分别为y#710; = 1.785×10-5D2 + 1.174×10-3D + 1.054和y#710; = 3.538×10-5D2 + 8.338×10-4D + 1.032。 ［结论］ 氡及其子体能诱发大鼠外周血淋巴细胞和气管-支气管上皮细胞的HPRT基因突变，呈现一定的剂量-效应关系 关键词：氡及其子体；外周血淋巴细胞；气管-支气管上皮细胞；HPRT基因位点突变 Measuring the HPRT Gene Mutation in Rat after Exposure to Radon and Its DaughtersCUI Feng-mei，NING Ping，DUAN Rong-fang，Tong Jian*（School of Radiation Medicine and Public Health，Soochow University，Suzhou 215123，China） Abstract：［Objective］ The HPRT gene mutation was studied in rat after inhaling radon and its daughters using the multinucleated cells method. ［Methods］ 24 Wistar male rats were divided into 4 groups evenly by random. When the accumulated dose of Radon and its daughters reached to the expected dose，the rats were anesthetized to get the peripheral blood lymphocytes and trachea-bronchia epithelial cells. The HPRT gene mutation test was performed using the multinucleated cells method. ［Results］ The results of the HPRT gene test indicated that the mutation frequencies（MFs）of HPRT gene of the peripheral blood lymphocytes and trachea-bronchia epithelial cells of Radon inhaled groups were all higher than the control respectively（P 0.05）. ［Conclusion］ Radon could cause increasing of MFs-HPRT in rat peripheral blood lymphocytes and trachea-bronchial epithelial cells. Key Words：radon and its daughters；peripheral blood lymphocyte；trachea-bronchial epithelial cell；HPRT gene locus mutation 近年来，居室和职业氡暴露造成的危害不断引起各方的关注，并成为研究的热点。在对氡暴露进行危险度评价时，利用生物样品和生物指标进行剂量估算具有重要的实际意义。次黄嘌呤鸟嘌呤磷酸核糖转移酶（Hypoxanthine-guanine phosphoribosyl transgerase，HPRT）基因位点突变分析是一种潜在的辐射生物剂量计，常用的检测方法是多核细胞法［1］。本实验通过检测氡及其子体诱发的大鼠外周血淋巴细胞和气管-支气管上皮细胞的HPRT基因位点突变频率，拟合剂量效应曲线，为HPRT基因位点突变作为氡暴露的辐射生物剂量计提供依据 1 材料与方法 1.1 主要试剂 细胞松弛素-B（Cy