发光夜 南京医科大学(英文版).pdfVIP

Parasitol Res (2011) 108:417–423 DOI 10.1007/s00436-010-2082-9 ORIGINAL PAPER prag01 , a novel deltamethrin-resistance-associated gene from Culex pipiens pallens Jin Zhang Mifang Yang Weijie Wang Haibo Sun Yang Xu Lei Ma Yan Sun Changliang Zhu Received: 25 June 2010 /Accepted: 8 September 2010 /Published online: 5 October 2010 # Springer-Verlag 2010 Abstract The prag01 gene (GenBank accession no. data for the first time approved that prag01 gene might play EU073017) was cloned from Culex pipiens pallens. An open some role in the development of deltamethrin resistance in C. reading frame of 270 bp was found to encode a putative 89- pipiens pallens . amino-acid protein which has the highest homology with Culex quinquefasciatus and Anopheles funestus . Real-time quantitative PCR analysis demonstrated that the transcription Introduction level of prag01 gene in deltamethrin-resistant strain was 1.65-fold higher than in deltamethrin-susceptible strain of C. Insect-borne diseases such as malaria, dengue fever, yellow pipiens pallens . Overexpression of prag01 gene in the fever, filariasis, and West Nile fever remain a significant mosquito C6/36 cells showed better prolification than the problem in more than 100 countries worldwide (Moreira et al. cells with empty vector when treated by deltamethrin. Our 2009). The disease burden is high, with more than 500 million cases each year. Mosquito is the main vector of these Jin Zhang and Mifang Yang have contributed equally to this work. diseases, causing millions of cases and thousands of deaths J. Zhang :M. Yang :W. Wang :H. Sun :Y. Xu :L. Ma :Y. Sun : each year. Chemical control is a major method to manage the C. Zhu (*)