Cyclin A and Cyclin D1 中国医科大学(英文版).pdfVIP

Int. J. Mol. Sci. 2012, 13, 828-839; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Lewis y Regulate Cell Cycle Related Factors in Ovarian Carcinoma Cell RMG-I in Vitro via ERK and Akt Signaling Pathways Dawo Liu 1, Juanjuan Liu 1, Bei Lin 1,*, Shuice Liu 1, Rui Hou 1, Yingying Hao 1, Qing Liu 1, 1 2 Shulan Zhang and Masao Iwamori 1 Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China; E-Mails: cyldw2007@163.com(D.L.); juanjuanliu_lg@ (J.L.); liushui cc@ (S.L.); hour@126.com(R.H.); haoyy@163.com(Y.H.); qingLiu@126.com (Q.L.); zhangshl99@126.com (S.Z.) 2 Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan; E-Mail: iwamori@163.com * Author to whom correspondence should be addressed; E-Mail: linbei88@; Tel.: +86-24-96615-1-41511. Received: 16 December 2011; in revised form: 28 December 2011 / Accepted: 4 January 2012 / Published: 16 January 2012 Abstract: Objective: To investigate the effect of Lewis y overexpression on the expression of proliferation-related factors in ovarian cancer cells. Methods : mRNA levels of cyclins, CDKs, and CKIs were measured in cells before and after transfection with the α1,2-fucosyltransferase gene by real-time PCR, and protein levels of cyclins, CDKs and CKIs were determined in