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Chinese Medical Journal 2012;125(22):3985-3990 3985
Original article
Follicular fluid levels of prostaglandin E2 and the effect of
prostaglandin E2 on steroidogenesis in granulosa-lutein cells in
women with moderate and severe endometriosis undergoing in
vitro fertilization and embryo transfer
WANG Jing, SHEN Xin-xin, HUANG Xiang-hua and ZHAO Zhi-ming
Keywords: endometriosis; follicular fluid; prostaglandin E2; granulosa-lutein cells
Background The mechanisms of endometriosis with infertility have not been fully studied. The present study aimed to
assess the follicular fluid (FF) levels of prostaglandin E2 (PGE2), which plays a critical role within the ovary, and to
investigate the effect of PGE2 on steroidogenesis in granulosa-lutein cells (GLCs) from women with and without
endometriosis.
Methods Thirty-three women with laparoscopically documented endometriosis and 40 controls undergoing in vitro
fertilization (IVF) were studied. We assayed the concentrations of PGE2 in FF, the production of E2 and progesterone in
FF and in culture medium, and the expression of steroidogenic acute regulatory protein (StAR) and CYP19A1 in GLCs
with the intervention of PGE2.
Results PGE2 and progesterone concentrations were increased and displayed positive correlation in endometriotic FF.
PGE2 induced the expression of StAR and the production of progesterone in GLCs from women with endometriosis, and
the expression of StAR and the production of progesterone were increased in GLCs from women with endometriosis.
However, there were no significant effects of PGE2 on promoting the production of E2 or the expression of CYP19A1 in
GLCs. Moreover, the production of E2 and the expression of CYP19A1 in GLCs from w
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