MMP-9, NM23-H1 and E-Cadherin 重庆医科大学(英文版).pdfVIP

Mol Cell Biochem (2011) 349:83–95 DOI 10.1007/s11010-010-0663-7 Knockdown of ribonuclease inhibitor expression with siRNA in non-invasive bladder cancer cell line BIU-87 promotes growth and metastasis potentials Junxia Chen • Xi Ou-Yang • Juan Gao • Jun Zhu • Xiaoyan He • Jiang Rong Received: 3 April 2010 / Accepted: 15 November 2010 / Published online: 2 December 2010 Springer Science+Business Media, LLC. 2010 Abstract Human ribonuclease inhibitor (RI) is a cyto- showed a significant facilitation of the tumor with heavier plasmic acidic protein. RI is constructed almost entirely of tumor weight, higher density of microvessels, lower nm23- leucine-rich repeats, which might be involved in some H1 and E-Cadherin expressions than those in the control unknown biological functions like other structurally similar group. Taken together, these experiments suggest that proteins besides inhibiting RNase A and angiogenin knockdown of RI could promote growth and metastasis activities. Our previous experiments demonstrated that up- potentials of BIU-87 cells. Our present findings reveal the regulating RI might effectively inhibit some tumor growth novel mechanism that anti-tumor effect of RI is also and metastasis. However, the down-regulating RI influence involved in suppressing growth and metastasis, besides on the tumor does not have any report until now, the antiangiogenesis. The results show that RI may be a ther- mechanisms underlying antitumor of RI have not been apeutic target protein for bladder cancer and may be of fully understood. In this study, the efficient RNA inter- biological importance. ferences of RI were constructed using a plasmid vector and identified with RT-PCR, Western blot and Immunocyto-