成年生长激素缺乏动物模型与骨代谢相关研究创新.docxVIP

成年生长激素缺乏动物模型与骨代谢相关研究袁人飞1,2,邓伟民2*,韩丽萍2,陈小香2,3,苏海容2,3（1. 广东药学院，广州 510006；2. 广州军区广州总医院，广州　510010；3. 广州中医药大学，广州 510403）【摘要】目的探讨近年来成年生长激素缺乏动物模型的几种建立方法，为实验研究和治疗因生长激素缺乏引起的骨代谢异常提供良好的模型。方法通过查阅文献，对成年生长激素缺乏动物模型的建立方法进行综述和评价。结果成年生长激素缺乏动物模型可分为自发性生长激素缺乏动物模型、垂体切除动物模型、基因敲除模型三种。结论垂体切除动物模型价格低廉，可操作性强，但受影响的因素较多，不适合于生长激素与骨代谢之间关系的研究；自发性生长激素缺乏动物模型与基因敲除动物模型价格高昂，但特异性缺乏生长激素，利于研究生长激素缺乏后，骨代谢的影响。【关键词】生长激素缺乏；动物模型；骨代谢；胰岛素样生长因子1Related research of animal model of adult growth hormone deficiency and bone metabolismYUAN Ren-fei1,2, DENG Wei-min2*, HAN Li-ping2, CHEN Xiao-xiang2,3，SU Hai-rong2,3（1.Guangdong Pharmaceutical University,Guangzhou 510006，China；2.General Hospital of Guangzhou Military Command of Chinese PLA,Guangzhou 510010,China；3.Traditional Chinese Medicine University of Guangzhou ,Guangzhou 510403,China骨代谢）【Abstract】Objective To explore the establishment methods of animal models of adult growth hormone deficiency, and to provide a good model for experimental research and treatment for abnormal bone metabolism caused by growth hormone deficiency.MethodsThe establishment methods of animal models of adult growth hormone were reviewed and evaluated by consult literature.Results There are three method which includespontaneous lack, pituitectomy and gene knockout can establish the animal model of adult growth hormone deficiency. Conclusions Hypophysectomizedanimal modelsis inexpensive and easy to create, butnotsuitable for studyingthe relationship betweengrowth hormoneand bone metabolism.Spontaneous lack and gene knockout modelsare specificgrowth hormone deficiency and of great research significance indiscussing the relationship between growth hormone andbone metabolism.【Key words】Growth hormone deficiency; Animal model; Bone metabolism; Insulin-like growth factor-I[项目基金] 军队保健专项项目（编号：13BJZ13）。[作者简介] 袁人飞（1992- ），男，硕士研究生。Email：yuanrenfei@139.com。[通讯作者] 邓伟民（1959- ），男，教授，硕士。Email：dengweimin1959@21。代谢性骨病是指机体因先天或后天性因素破坏或干扰了正常骨代谢和生化状态，导致骨生化代谢障碍而发生的骨疾患。整个生命过程中，生长激素（growth hormone， GH）与胰岛素样生长因子-1（ins