槲皮素对黄粉虫血淋巴酚氧化酶的生理效应.pdfVIP

昆 虫 学 报 Acta Entomologica Sinica，December 2007，50(12)：1201—1206 1SSN 0454—6296 槲皮素对黄粉虫血淋巴酚氧化酶的生理效应 刘守柱，肖 婷，薛超彬，罗万春 (山东农业大学植物保护学院，农药毒理与应用技术省级重点实验室，山东泰安 271018) 摘要：用酶标仪法测定了槲皮素对黄粉虫 Tenebrio molitor酚氧化酶(phenoloxidase，PO)的生物活性。结果表明：在离 体条件下，对黄粉虫血淋巴P0的lc 为0．625 mmol／L。在活体情况下，当槲皮素一DMSO溶液或槲皮素水悬浮液(5 L)注入虫体后，在浓度低于1．0 mmol／L时对黄粉虫血淋巴P0具有激活作用，当浓度高于2．0 mmol／L时则对P0具 有明显的抑制作用；单独注射5 L DMSO溶液对 P0活性亦有一定的激活作用。试虫被注射槲皮素后，P0活性在2 ～ 4 h内迅速下降，然后缓慢上升，处理8 h左右达到最高点，其后低浓度处理 P0活性保持不变，而高浓度处理则逐 渐下降，提示低浓度槲皮素可以引起试虫的免疫反应。在P0测活体系中加入0．5％的BSA后对P0活性无影响，并 能使槲皮素在测活体系中保持稳定。 关键词：黄粉虫；槲皮素；酚氧化酶；生理效应 中图分类号：Q965 文献标识码：A 文章编号：0454—6296(2007)12—1201—06 Physiological effect of quercetin on phenoloxidase from Tenebrio mofitor LIU Shou·Zhu，XIAO Ting，XUE Chao·Bin，LUO Wan·Chun (College of Plant Protection，Shandong Key Laboratory of Pesticide Toxicology and Application Technique，Shandong Agricultural University，Tai’an， Shandong 271018，China) Abstract：The physiological effect of quercetin on phenoloxidase(PO)from TenebKo molitor was determined in itro and in vivo．The results showed that the inhibition concentration showing 50％ of the maximum inhibition(IC50)was 0．625 mmol／L．1’he PO activity of hemolymph in vivo Was increased when the quercetin· DMSO solution or quercetin．water suspension(5／,L)with concentration of0．2—1．0 mmol／L Was injected into the larvae of T．molitor；but the compound would decrease the PO activity when the concentration of quercetin was above 2．0 mmol／L．In addition，the PO activity was also increased when DMSO(5／,L)was injected into the tested insect in vivo．During 2—4 hours after injection of quercetin into the body of the insect，the PO activity was decreased rapidly，then went slowly up and reached the highest level 8 h after injection of the compound．and then dropped again except the low concentr