芦苇生物活性组分对小球藻生长的促进效应.pdf

中国环境科学 2009,29(11) ：1186~1189 China Environmental Science 芦苇生物活性组分对小球藻生长的促进效应 郭长城,李 鑫,胡洪营* (清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京 100084) 摘要：利用乙醇从芦苇(Phragmites australis)生物质中提取得到具有生物活性的粗提液,研究了芦苇乙醇粗提液对蛋白核小球藻生物量、叶 绿素含量、蛋白质含量的影响.结果表明,芦苇中存在的生物活性组分对蛋白核小球藻的生物量、叶绿素含量、蛋白质含量的提高均具有 促进效应,层析中性组分可使小球藻藻密度平均提高 0.87 倍,最高可提高 1.79 倍,叶绿素含量及蛋白质含量平均提高 0.67 倍和 0.99 倍,最高 可提高 2.71 倍和 4.76 倍.芦苇中存在的高价值生物活性物质具有很好的开发及应用潜力. 关键词：芦苇；活性组分；蛋白核小球藻；促进效应 中图分类号：X17 文献标识码：A 文章编号：1000-6923(2009)11-1186-04 Enhancing effect of Phragmites australis bioactive substances on the growth of Chlorella pyrenoidosa. GUO * Chang-cheng, LI Xin, HU Hong-ying (State Joint Key Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China). China Environmental Science, 2009,29(11)：1186~1189 Abstract ：Bioactive substances were extracted from Phragmites australis by using ethanol and the enhancing effects on biomass, chlorophyll content and protein content of Chlorella pyrenoidosa was studied. The bioactive substances exited in Phragmites australis had strong promoting effect on biomass, chlorophyll content and protein of Chlorella pyrenoidosa. Among neutral fractions of ethanol, the density of Chlorella pyrenoidosa was improved about average of 0.87 times and the maximum increase was 1.79 times. The content of chlorophyll and protein were improved by 0.67 times and 0.99 times averagely, the maximum increase were 2.71 and 4.76 times. The high-value bioactive substance has a good potential for development and application. Key words ：Phragmites australis bioactive substances Chlorella pyrenoidosa enhancing effect 蛋白核小球藻(Chlorella pyrenoidosa) 是淡 的禾本科湿地植物.本试验利用从山东省南四湖 水绿藻中的单细胞微藻,在生长发育过程中,合成 采集的芦苇,提取并分离纯化,得到了具有生物活 的脂肪酸类物质具有潜在的生物燃料价值, 因而