兔耳软骨创伤愈合的非线性光谱成像研究.pdfVIP

目录 中文摘要··························································1 英文摘要··························································2 缩略词····························································3 1 前言···························································4 1.1 耳软骨创伤愈合概述·············································5 1.2 双光子显微术的概述 ············································10 2 实验材料与方法··············································15 2.1 实验材料与仪器················································15 2.2 试验方法 ······················································15 2.3 观察指标 ······················································17 2.4 统计分析······················································18 3 结果···························································18 3.1 无损耳软骨组织非线性光谱成像下的形态结构······················18 3.2 不同愈合时期耳软骨创伤修复组织非线性光谱成像··················18 3.3 不同愈合时期耳软骨胶原密度、胶原方向、软骨陷窝横切面积定量及相互比 较。·······························································21 4 讨论 ··························································24 5 结论··························································28 3 万方数据 兔耳软骨创伤愈合的非线性光谱成像研究 【摘要】 【目的】试图利用基于双光子激发荧光联合二次谐波产生的非线性光谱成像 技术对兔耳软骨创伤愈合过程进行动态监测。探索一种在临床上能够对软骨创伤 愈合监测、软骨相关疾病诊断的一种新方法。 【方法】 建立兔耳软骨创伤愈合模型，于三个时间点即创伤后1 周、4 周、 20 周切取少许创伤边缘修复组织及对侧健康耳软骨标本做切片行非线性光谱成像 观察及 HE 染色观察。重点观察修复组织基本形态变化、胶原含量变化、胶原方 向改变、软骨陷窝大小变化。 【结果】 1、非线性光谱成像技术成功实现了对耳软骨组织进行高分辨的成 像，并且其所成图像与传统的组织切片HE 染色图像相吻合。2 、本技术还成功监 测到软骨创伤愈合过程中胶原含量的变化、胶原方向的变化、软骨陷窝大小的变 化。 【结论】 1、非线性光谱成像技术能够对耳软骨组织进行良好的成像，并且 对其中部分成分如胶原蛋白等进行相对定量检测。2 、软骨组织可以再生，但是新 生软骨组织在形态结构及基质成分含量上均有一定差异。3、本技术在临床上是一