RNAi在功能基因组学研究中的应用.ppt

* Conditional RNAi strategies using the tetR/O system. * application Gene function analysis Modeling of diseases Developmental defects caused by shRNA-mediated gene silencing * PNAS. 2006.2252–2256. * * Validate APC/Wnt and p19ARF as potential therapeutic targets in T cell acute lymphoblastic leukemia/lymphoma and lung adenocarcinoma, respectively. * * identified and validated 13 tumor suppressor genes, 12 of which had not been linked to cancer before. * The RNAi libraries database * /rnai-libraries * http://genomernai.dkfz.de/GenomeRNAi// RNAi应用中的问题 稳定性的问题 导入途径的问题 安全性问题 * * 它可以用于研究单基因功能或基