辛基酚的好氧生物降解及微生物群落特征-环境工程学报.DOCVIP

【拟再审修改稿辛基酚的好氧生物降解及群落 刘易(1上海环境保护有限公司,上海市环境科学研究院,上海 200233; 2长江水环境教育部重点实验室,同济大学,上海 200092) 摘要：利用典型的活性污泥试验了辛基酚（Octylphenol, OP）的生物降解性能。研究了水力停留时间、OP浓度和碳氮比对OP处理效率的影响，通过Real-Time PCR和PCR-DGGE方法分别分析了微生物群落的总体密度和多样性变化。通过30天的启动， OP在序批式活性污泥典型好氧条件下出现有效生物降解。在OP浓度50～150μg/L整个试验过程中，OP去除率基本保持稳定。在试验范围内，较长的水力停留时间（12h）和碳氮比（2:1）有利于OP生物降解。通过Real-Time PCR系统微生物平均密度约为1.22g/L，但富碳状态下生物密度增长上升。通过DGGE分离到17，包括了α-proteobacteria（6）、β-proteobacteria（5）、γ-proteobacteria（2）、Actinobacteria（2）和δ-proteobacteria（1）群落。α-proteobacteria和δ-proteobacteria菌群密度呈现增长，β-proteobacteria和Actinobacteria菌群中的部分种类密度出现减少，OP在活性污泥中的好氧生物降解可能与α-proteobacteria中的特定类别相关。关键词Octylphenol；活性污泥好氧生物降解α-proteobacteria 中图分类号X172 文献标识码A 文章编号 Aerobic octylphenol bio-degradation in activated sludge and characteristic of microbial community. Liu Yi1, Wang Feng2* (1Shanghai Environmental Protection Limited Company, Shanghai Academy of Environmental Sciences, Shanghai 200233, China; 2Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Shanghai 200092, China) Abstract： activated sludge process was employed to investigate the influence of HRT, initial OP concentration and C/N on aerobic OP biodegradation. Real-Time PCR and PCR-DGGE methods were applied respectively to analysis the variety in population concentration and bio-diversity of the bacterial community. After 30 days start-up, an effective OP biodegradation appeared in the lab-scale sequencing batch reactor (SBR) under aerobic conditions. With influent OP concentrations of 50 ~ 150μg/L throughout the entire experiment, OP removal rate remained relatively stable, ranging from 94 to 99%. It was demonstrated that a long HRT (12h) and low C/N (2:1) was beneficial for OP biodegradation. The mean population concentration of the bacterial was about 1.22g/L, however it seemed to increase at carbon-riched condition. 17 dominant bacterial strains were separated from DGGE gel, which were divided into α-proteobacteria(6 strains), β-proteobacteria(