RP-HPLC法测定犬血浆中奈诺沙星浓度.pdfVIP

.671. 中国抗生素杂志2016年9月第41卷第9期 文章编号: 1 ∞1-8689(2016)09-0671-04 即-HPLC法测定犬血浆中奈诺沙星浓度 唐炯1 程强1 • 孙文霞1 田娱l 张羽2 (1 抗生素研究与再评价四川省重点实验室，四川抗菌素工业研究所，成都大学，成都 610052; 2 成都盛迪医药有限公司，成都610∞0) 摘要:目的 采用HPLC法测定比格犬血浆中噎诺嗣类药物奈诺沙星的浓度。方法 以莫西沙星作为内标，血浆样品经 二氯甲兢萃取后，采用HPLC法进行测定，色谱柱为Welchrom DOS-C 18(200mmX6mm, 5μm)，流动相为乙腊·水(含0.2%三乙 肢，磷酸调pH2.8)(22:78) ，检测波长292nm，流速1 mL/min ，柱温40C。结果 血浆中奈诺沙星的线性范围为0.1-12.0J.lglmL (1哇。.9996)，平均方法回收率为101.25%，日内RSD8.0%，日间RSD8.5%。结论 所用方法的专属性强、准确、灵敏、快速 简便，适用于测定血浆中奈诺沙星的浓度。 关键词2 反向高效液相色谱法:噎诺酣类:奈诺刻，、星:血药浓度 中图分类号: R9 17 文献标志码:A Determination of nemonoxacin in dog plasma by RP-HPLC l l 1 l 2 Tang Jiong , Cheng Qiang , Sun Wen-xia , Tian Yuan 扭dZhangYu (1 Antibiotics Research and Re-evaluation Key Laboratory ofSichuan Province, Sichuan Industrial Institute ofAntibiotic, Chengdu University, Chengdu 610052; 2 Chengdu Suncadia Phannaceuticals Co., Ltd, Chengdu 610∞0) Abstrad Objective To establish an HPLC method for determination of nemonoxacin in beagles plasma. Methods Moxifloxacin was used as intemal standard,the plasma samples were pretreated by dichloridimethane, and then analyzed by x HPLC. The HPLC was performed on a Welchrom DOS-CI8 column (200mm 4.6mm, 5阳n)with uv detection at 292nm, using phosphoric acid solution (containing 0.2% triethylamine ,a司justed to pH2.8 with phosphoric acid) and acetonitile (78:22) as mobile phase at 1.0mL/min flow rate,And the column tempe