PITC柱前衍生高效液相色谱法测定接骨木果实中氨基酸含量.pdfVIP

贵州农业科学 2015 ， 43(的 :213~216 Guizhou Agricultural Sciences [文章编号]1001-3601( 201日08-0457-0213-04 PITC 柱前衍生高效液相包谱法测定接骨木果实中氨基酸含量 于洪超1 ，马荣申2 ，郭庆梅1\ 周凤琴1 ，郭俊国3 (1.山东中医药大学药学院，山东济南 250355; 2. 济南天红茵木种植技术研发中心，山东济南 250400; 3. 山东中医药大学眼科研究所，山东济南 250002 ) [摘 要]为了测定接骨木果实中游离氨基酸和水解氨基酸含量，采用 PITC 柱前衍生高效液相色谱法 (RP-HPLC) 以异硫氯酸苯酶(PITC)作柱前衍生试剂，采用 RP-HPLC 梯度洗脱的方法测定接骨木果实中 的游离和水解氨基酸的峰面积并计算其含量。结果表明:接骨木果实中氨基酸和水解氨基酸的含量分别为 2.107%和 7.297% ;1 8 种氨基酸分离良好，线性关系良好，空白无干扰，精密度 RSD 在 o. 634% ~2. 532%. 稳定性 RSD 在 O. 729%~2. 774%. 重复性 RSD 在 O. 664%~2. 884%. 平均回收率在 95. 49%~103. 67%. 且 RSD 均小于 3% 0 RP-HPLC 法灵敏、简便、快捷，能准确测定接骨木果实中游离氨基酸和水解氨基酸的 含量。 [关键词]接骨木;游离氨基酸;水解氨基酸;异硫氯酸苯醋;高效液相色谱法 [中图分类号] S567 [文献标识码]A Determination of Amino Acids in the Fruits of Sαmbucus williαmsii by PITC Pre-column Derivation and RP-HPLC Method 1 2 篝 1 3 YU Hongchao • MA Rongshen • GUO Qingmei1 • ZHOU Fengqin • GUO Jungu0 (1. Shandong University 0/ Traditional Chinese Medicine , J inan , Shandong 250355; 2. J inan Tianhong Seedling Planting Technology Research and Development Center , Jinan , Shandong 250400; 3. Eye lnstitute , Shandong U- niversity 0/ 町M ， Jinan , Shandong 250002 , China) Abstract: For the determination of free and hydrolysis amino acids in the fruits of S. williamsii , taking phenyl isothiocyanate (PITC) as the pr e-column derivating reagent , RP- HPLC gradient elution method was employed to measure the peak area and contents of free and hydrolytic amino acid. Results: Contents of free amino acid and hydrolytic amino acid in the fruits of S. williamsii were 2. 107 % and 7.297 % respectively. separation of 18 kinds of amino acids was good. the linear relationship was good , and the blank had