RP-HPLC法测定鹿阴道硅胶栓体外模拟释放的醋酸氟孕酮.pdfVIP

特产研究 Special Wild Economic Animal and Plant Research 43 文章编号:1ω1-4721(2011 刷4刷3-04 RP-HPLC 法测定鹿阴道 硅胶栓体外模拟释放的醋酸氟孕酣 王玉方 1 ，商云帅 1 ，张杰 l ，王晓霞 2 (1.中国农业科学院特产研究所，吉林吉林 132109; 2. 吉林省磐石市动物疫病预防控制中心，吉林磐石 1323∞) 摘要:建立鹿用黯酸氟孕嗣阴道硅胶栓在以水为介质、恒温气浴摇床振摇的体外模拟释放环境下水溶液中醋酸氟孕嗣含量测 定的反相高效被相色谱法。分析柱为Waters SunFire田CI85 川(内径4.6翩，长 15Omm) ，柱温3OC，流动相为甲醉·水，体积 比为7:3 ，等度洗脱，流速0.7mUmin， PDA 检测波长242nm，进样量 20μL。醋酸氟孕酣在 14min 内在得完全分离，黯酸氟孕嗣标 准品在O.∞83-1.6问的进样范围内，响应值和进样量间呈现优良的线性关系。当信噪比为3 时，检出限为0.056μ IifmL。该方 法简便、可靠，适应性强。 关键词:醋酸氟孕酣;测定;体外模拟;RP-HPLC; PDA 中固分类号: Q503 文献标识码:A Determination of Flurogestone Acetate for in Vitro Simulated Release Solution of D创r s Intravaginal Silica Gel Forelock by RP-HPLC Method l l l 2 WANG Yu-fang , SHANG Yun-shuai , ZHANG Jie , WANG Xiao-xia (l .1nstitute of S阳ial Wild Economic Animals and Plants, CAAS, Jilin 132109, China; 2. Animal Dis倒臼Prevention and Control Center in Panshi City, Jilin Province, Panshi 1323∞，China) Abstract: To 四tahlish a RP-HPLC method for the f)w吨创抽IIJe acetate determinatiOll in vitro sÎmulated reI回øe 801四011 (wa加r. the n时ium，白白 constant tem阳曲Jre shaking bed) of deer s in国V咱nal silica gel forelock. Wi由 aSunFir俨CI85 件m column 制4.6 mm， le吨由 150 mm) , column 棚nperature maintainP.d at 30飞mobile phaøe isωm阳p.d ofme由础。，1 and water, the volume ratio of阳也anolω water is 7:3 , the column is eluted at a flow rate of 0.7 mUmin by 回到[!I由c me曲。d， PDA detection wavelength maintained at 242 nm, a 20μL in,iect volume.ηle mP.thod could 回归.rate FGA pert;创tly 响曲in 14 minutes. and gave the excellfmt lin创r relationship úom O.创)8 3μ.g to 1.6μg FGA amount. When the ratio of signalωnoiøe is 孔险nit of detection reached