pGL4.19哺乳动物表达载体说明.pdfVIP

pGL4.19 编号 载体名称 北京华越洋生物VECT6045 pGL4.19 pGL4.19载体基本信息 载体名称: pGL4.19 、pGL4.19[luc2CP/Neo] 质粒类型: 哺乳细胞表达；信号通路报告载体 高拷贝/低拷贝: -- 启动子: 无启动子 克隆方法: 多克隆位点，限制性内切酶 载体大小: 5778 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: -- 载体抗性: 氨苄青霉素Ampicillin 筛选标记: 新霉素Neomycin pGL4.19载体不含启动子，带有高度去稳定性的荧光素酶报告基因，用于研 备注: 究目的启动子和增强子的活性。 稳定性: -- 组成型: 组成型 病毒/ 非病毒: 非病毒 pGL4.19载体质粒图谱和多克隆位点信息 pGL4.19 载体简介 The pGL4.19[luc2CP/Neo] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression. The pGL4.19[luc2CP/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart. pGL4.19 载体序列 ORIGIN 1