在PSGL-1缺失基因工程小鼠外周血中IGFBP6和TNF-αmRNA表达的研究.DOC

研究报告 PSGL-1敲除小鼠血中MIP-1γ和TNF-α的表达 李江超，周泽启，叶杰，韩露，叶宇翔，刘影，何晓东，陈卉，袁俏冰，黎帅，张静丽，王丽京 （广东药学院 血管生物学研究所，广东 广州 510006） 【摘要】 目的 研究PSGL-1缺失对遗传基因工程小鼠外周血血常规的影响，并检测以及外周血中炎症因子IGFBP-6、TNF-α和MIP-1γmRNA表达的水平。 方法 用血常规检测方法检测正常C57/BL/6小鼠和PSGL-1基因缺失的基因工程小鼠（PSGL-1-/-小鼠）的外周血中血常规的差异；其次，提取两种鼠的血液的mRNA，逆转录为cDNA，采用eal-time PCR方法，检测C57小鼠与PSGL-1-/-小鼠外周血中炎症因子 TNF-α和MIP-1γmRNA的差异。结果 与对照组C57小鼠相比，PSGL-1-/-基因工程小鼠在12周龄时外周血中中性粒细胞（*P0.05）、淋巴细胞(**P0.01)和白细胞细胞（***P0.001）总数显著增加和炎症因子TNF-α以及MIP-1γ表达增加(P0.05)。结论 PSGL-1的缺失改变了小鼠细胞因子影响了外周血的血细胞组成，MIP-1γ和TNF-α炎症因子上调，有可能影响了小鼠的免疫功能。 【关键词】PSGL-1；TNF-α；MIP-1γ，基因工程动物 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.002.000 Expression of MIP-1γ and TNF-α in PSGL-1 knockout mice LI Jiang-chao，ZHOU Ze-qi, YE Jie, HAN Lu, YE Yu-xiang, LIU Ying, HE Xiao-dong, CHEN Hui, YUAN Qiao-bing, LI Shuai, ZHANG Jing-li, WANG Li-jing (Institute of Vascular Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China) 【Abstract】 ObjectivePSGL-1 on routine blood test results in PSGL-1 knockout (PSGL-1-/-) mice, and to detect the expression levels of cytokines IGFBP-6, TNF-α and MIP-1γ mRNA in their peripheral blood. Methods To detect the differences in routine blood test results of normal C57/BL/6 mice and PSGL-1-/- mice. To prepare cDNA from isolated blood mRNA, and to analyze the differences in expression of cytokines TNF-α and MIP-1γ mRNA in peripheral blood of the C57 mice (control) and PSGL-1-/- mice by real-time PCR. Results Compared with the C57 control mice, the 12-week old PSGL-1-/- mice showed significantly increased neutrophil (P0.05), lymphocyte (P0.01) and total leukocyte counts (P0.001), and increased expression levels of TNF-α and MIP-1γ mRNA in the peripheral blood (P0.05). Conclusions Our findings suggest that the loss of PSGL-1 changes the cell counts of peripheral blood in mice. The up-regulation of cytokines MIP-1γ and TNF-α may influence the immune function of the mice. 【Key Words】 PSGL-1;