荠菜多糖的分离纯化与单糖组成分析.pdfVIP

82 Medical Science Journal of Central South China,January2015,Vol.43,No.1 DOI:10郾15972/ j郾cnki郾43鄄1509/ r郾2015郾01郾021 ·技术与方法 · 荠菜多糖的分离纯化与单糖组成分析 1 2 2 2 2 王摇 华 ,熊淑娟 ,曹摇 轩 ,陈临溪 ,唐国涛 (1郾 南华大学附属第一医院药剂科,湖南衡阳421001;2郾 南华大学药物药理研究所) 摘摇 要:摇 目的摇 对荠菜多糖进行分离纯化并分析其单糖组成。 摇 方法摇 采用超声法提取总多糖,除蛋白,用 SephadexG鄄75进行纯化,水解后用气相色谱分析其单糖组成。 摇 结果摇 分离得到两个多糖组分1和2。 气相色谱 鉴定出其中四种单糖组成,D鄄木糖、L鄄鼠李糖、D鄄甘露醇和D鄄葡萄糖,其组成比例为1:1.8:270.8:1。 摇 结论摇 从荠 菜多糖中分离出两种多糖组分,组分2 由8种多糖组成,鉴定出其中4种。 关键词:摇 荠菜多糖;摇 分离纯化;摇 单糖组成;摇 气相色谱 中图分类号:R917摇 摇 摇 文献标识码:A Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharide from Shepherdspurse Herb WANG Hua,XIONG Shujuan,CAO Xuan,et al (The FirstAffiliated Hospital,University of South China,Hengyang,Hunan421001,China) Abstract:摇 Objective摇 to isolate andpurify thepolysaccharide and analyzethe monosaccharide composition.摇 Methods 摇 thepolysaccharideextractionremovingtheproteinswerepurifiedthrough sephadex G75,thepolysaccharide componentswere analyzedby gas chromatography(GC)after hydrolization.摇 Relsults摇 two monosaccharide componentswere obtained after purification.Four monosaccharide compositions concluding D鄄xylose,L鄄rham鄄 nose,D鄄mannose and D鄄glucosewere measuredby GCinthe secondcomponentsandtheproportionwere 1颐 1.8颐 270.8颐1.摇 Conclusion摇 two polysaccharidefractionswereisolatedfromShepherdspurse Herb.The sec鄄 ondfractionwascomposed of eight kindsof monosaccharide andfour of themwere identified. Key words:摇 Polysaccharide of Shepherdspurse Herb;摇 isolation and purification;摇 monosaccha鄄 ride compositions;摇 gas chromatography(GC)