人血浆中二羟丙茶碱的HPLC法测定.pdfVIP

中国医药工业杂志 Chinese Journal of Pharmaceuticals 2013 , 44 (1 0) • 1033 • 人血浆中二起丙茶碱的 HPLC 法测定 巩克民I ，王宁I ，陈钧I ，季宏建2 ，杨友回1* (1.盐城卫生职业技术学院，江苏盐城 224005; 2. 盐城市第三人民医院，江苏盐城 224001) 摘要:建立了高效液相色谱法测定人血浆中的二经丙茶碱。以咖啡因为内标，色谱柱为 Elite SinoChrom ODS AP C8 柱， 以甲醇-水 (22 : 78) 为流动相，检测波长273 nm。二短丙茶碱在 5.2 ~ 260.0 Ilg/ml 范围内线性关系良好:方法回收率 为 97.71%~ 99.23% ，提取回收率为 90.58%~ 92.56% ，日内和日间RSD 分别为 5.19% ~ 8.1 9%和1.53%~ 8.49% 。 关键词:二泾丙茶碱;高效液相色谱:测定 中图分类号: 0657.7+2 文献标志码:A 文章编号: 1001-8255 (2013) 10-1033-03 Determination of Diprophylline in Human Plasma by HPLC 2 GONG Kemin\ WANG Ning\ CHEN Jun\ JI Hongjian ,YANG Youtian* (I. Yancheng Institute 01Health Science, Yancheng 224005; Yancheng ThirdPeoples Hospital, Yancheng 22400 1) ABSTRACT: An HPLC method was established for the determination of diprophylline in human plasma. Caffeine was used as the intemal standard. An Elite SinoChrom ODS AP CI8 column was used , with the mobile phase of methanol- water (22 : 78) at the detection wavelength of 273 nm. 白白 calibration cu凹e was linear in the range of 5.2 - 260μg1ml. The method recoveries were 97.71 % - 99.23 % and the extraction recoveries were 90.58% - 92.56%. The intra- and inter- day RSDs were 5.1 9% - 8.1 9% and 1.53% - 8.49% , respectively. Key Words: diprophylline; HPLC; determination 二起丙茶碱 (diprophylline， 1) 系茶碱与→氯 1，方法简单快速，结果准确可靠，可用于 1 常规 丙二醇缩合的黄嗦岭衍生物，临床用作平滑肌松弛 治疗时的血药浓度监测。 药，用于支气管哮喘、喘息型支气管炎和阻塞性肺 1 仪器与试药 Elite 230 型高效液相色谱系统，含两元P230 11 型高压 气肿等以缓解喘息症状，也用于心源性肺水肿引起