华山松松塔多糖中单糖组分的HPLC法分析及测定.pdfVIP

中国医药工业杂志 Chinese Journal of Pharmaceuticals 2013 , 44 (2) 189 • 华山松松塔多糖中单糖组分的 HPLC 法分析及测定 张海珠，周萍气刘光明，夏从龙，史志婷 (大理学院药学院，云南大理 671000) 摘要z 建立了高效液相色谱法分析并测定华山松松塔多糖中的单糖。以三氟乙酸水解华山松松塔多糖，水解物加入 1- 苯 基-3- 甲基 -5- ~比唾酣 (PMP) 进行衍生化。使用 Agilent Venusil XBP-CI8 色谱柱，以乙睛-0.05 mo旺乙酸绞缓冲液 (pH 5.5) (20 : 80) 为流动相，检测波长250 nm。华山松松塔多糖由 10 种单糖(其中 3 种未鉴定)组成。己鉴定出的 7 种单糖均 在 0.005 - 0.2 mg/ml 范围内线性关系良好，回收率为 89.2%- 10 1.6% , RSD3.0% 。在华山松松塔提取物C、 D、 E 和 F 中的 D- 甘露糖、鼠李糖、 D- 葡萄糖隆酸、 D- 半乳糖醒酸、葡萄糖、半乳糖和 D- 阿拉伯糖的相对平均含量分别为 0.144%- 1.24% 、 0.13%- 2.14% 、 0-0.99% 、 0.17%- 2.27% 、 0.76%- 2.73% 、 0.14%- 9.17%和 0.16%- 8.16% 。 关键词z 华山松松塔多糖:单糖:衍生化:高效液相色谱:测定 中图分类号: 0657.7+2 文章编号: 1001-8255(2013)02-0189-03 文献标志码:A Analysis and Determination ofMonosaccharide Composition in Polysaccharides from Pinecone ofPinus armandi Franch. by HPLC ZHANG Haizhu , ZHOU Ping* , LIU Guangming, XIA Conglong, SHI Zhiting (College 01Pharmacy, Dali University, Dali 671000) ABSTRACT: An HPLC method was established for the analysis and determination of the monosaccharides in polysaccharides from pinecone of Pinus armandi Franch.. The sample was hydrolyzed by trifluoroacetic acid and the hydrolysate was derivatized by reaction with l-phenyl-3-methyl-5-pyrazolone (PMP). An Agilent Venusil XBP-C I8 column was used with the mobile phase of acetonitrile-0.05 mol/L ammonium acetate buffer(pH 5.5) (20: 80) at the detection wavelength of 250 nm. The polysaccharides were composed of ten monosaccharides (including three unidentified monosaccharides). The calibration curves for the seven identified monosaccharides were linear in the range of 0.005 - 0.2 mglml. Th