雷公藤多甙对急性移植物抗宿主病小鼠的作用论文.docVIP

　　雷公藤多甙对急性移植物抗宿主病小鼠的作用论文 于艳秋，张义侠，金玉楠，卢晓梅，王巍，张海鹏 【摘要】 本研究应用雷公藤多甙防治小鼠急性移植物抗宿主病。对受致死量照射的C57BL/6受鼠，注入供鼠BABC/c骨髓与脾淋巴细胞的混合液，并给予雷公藤多甙、环孢素A、甲氨蝶呤处理。用免疫组织化学法、流式细胞术和ELISA法检测各组小鼠T淋巴细胞、黏附分子、细胞因子。结果表明： 所有异基因对照组小鼠.freelRNA的表达（P 0.05）；上调血清中IL-10的水平（P 0.05），但对IL-4的作用不显著（P 0.05）。结论：雷公藤多甙具有明显抗aGVHD作用并同时保留抗白血病效应，其作用机制与调节促炎/抑炎细胞因子的分泌和黏附分子的表达，以及抑制T淋巴细胞作用有关。 【关键词】 雷公藤多甙；移植物抗宿主病； T淋巴细胞； 黏附分子；细胞因子 Glucosidorum Tripterygii Tororum (GTT) is a kind of Chinese traditional medicine (CTM), for the first time used in China as an immunoregulatory agent ent of autoimmunological diseases such as rheumatoid arthritis (RA) and nephrotic syndrome. Our previous experiment shophocytes, inhibit the secretion of IL-2 and expression of IL-2R, doolecules (CD11a, CD18) on the surface of T cells. And it can prolong the survival time of allogeneic transplanted skin. Its effect on anti-rejection is ethylprednisolone. And,.freel shouch more stronger effect, indicating that GTT can moderately or obviously inhibit the function of T cells and the secretion of cytokines［1］. Furthermore, GTT may have anti-inflammatory and anti-tumor effects. Graft-Versus-Host disease (GVHD), especially acute Graft-Versus-Host disease (aGVHD) is a major lethal plication after bone marroed to adopt GTT or bine GTT echanism. Materials and Methods Mice Donor mice BALB/c(H-2d), female; syngeneic transplantation recipient mice BALB/c(H-2d), male; allogeneic transplantation recipient mice C57BL/6(H-2b) male. All of them y of Sciences). Induction of aGVHD The recipients received in, SIMENZ, Primus, Germany) for 6-8 hours before transplantation. Bone marroale BALB/c(H-2d) edium. Both BALB/c and C57BL/6 recipients arrophocytes via caudal vein. Assignment and process After transplantation, the recipients ly divided into follo. Their food and icin (320 mg/L) and erythromycin (250 mg/L) inistrated l; in CsA+MTX group g/kg everyday and MTX 7 mg/m2 on the day 1,3, 5 and 9; in GTT group g/kg; in GTT+CsA group g/kg and CsA 2.5 mg/kg. GTT