UPLCPDA同时测定藏药翼首草环烯醚萜苷及酚酸类10个化学成分含量.docVIP

UPLC??PDA同时测定藏药翼首草环烯醚萜苷及酚酸类10个化学成分含量 　　[摘要] 建立超高效液相色谱法（UPLCPDA）同时测定藏药翼首草环烯醚萜和酚酸类10个化学成分的含量。采用Acquity UPLCR BEH C18（2.1 mm ×100 mm，1.7 μm）色谱柱，流动相0.2%磷酸水（A）乙腈（B），梯度洗脱，检测波长237，325 nm，流速0.4 mLmin-1，柱温30 ℃，进样量1 μL。研究结果表明UPLCPDA法定量分析翼首草10个化学成分在考察的范围内线性关系良好（r≥0.999 7）；加样回收率为96.30%～103.0%（n=9），RSD为0.72%～2.9%（n=9）。所建立UPLC方法简便、准确、重复性好，可同时测定藏药翼首草中10个化学成分的含量 [关键词] 藏药； 翼首草； UPLCPDA； 环烯醚萜； 酚酸； 含量测定 Simultaneous determination of ten major compounds including iridoid glycosides and phenolic acids in Pterocephalus hookeri by UPLCPDA TANG Ce1， WEN Jian1， WANG Jing1， ZHAO Kehui1， FAN Gang1， MENG Xianli1， ZOU Zhongmei2， ZHANG Yi1* （1.College of Ethnic Medicine， Chengdu University of Traditional Chinese Medicine， Chengdu 611137， China； 2.Institute of Mechcinal Plant Development， Chinese Acadeuy of Medical Scieuce and Peking Union Medical College， Beijing 100193， China） [Abstract] This study is to develop an UPLCPDA method for determination of 10 major components in Pterocephalus. The UPLCPDA assay was performed on a Waters Acquity UPLCR BEH C18（2.1 mm ×100 mm，1.7 μm）， and the column temperature was at 30 ℃. The mobile phase consists of water containing 0.2% phosphoric acid （A） and acetonitrile （B） in gradient elution at a flow rate of 0.4 mLmin-1. The detection wave length was set at 237 and 325 nm， and the injection volume was 1 μL in the UPLC system. The linear range of 10 detected compounds were good （r≥0.999 7）， and the overall recoveries ranged from 96.30% to 103.0%， with the RSD ranging from 0.72% to 2.9%. The method was simple， accurate and reproducible， which can be used for the simultaneous determination of the content of ten major components in P. hookeri. [Key words] Tibetan medicine； Pterocephalus hookeri； UPLCPDA； iridoid glycoside； phenolic acid； content determination 翼首草（藏?Z名：榜孜毒乌）为川续断科匙叶翼首草Pterocephalus hookeri（C.B.Clarke）Heck 的干燥全草，收载于2015年版《中国药典》（一部）[1]及卫生部《藏药部颁标准》[2]中，为常用藏药。翼首草主要含有环烯醚萜类和皂苷类成分，如马钱苷、吴茱萸苷、齐墩果酸和熊果酸等[39]，具有抗病毒、抗氧化及提高免疫力等作用。课题组前