HNMR代谢组学技术用于牛黄解毒片中对雄黄ぞ哂信湮榧醵咀饔玫闹饕化学成分群研究.docVIP

1HNMR代谢组学技术用于牛黄解毒片中对雄黄?ぞ哂信湮榧醵咀饔玫闹饕?化学成分群的研究 　　[摘要] 利用1HNMR为基础的代谢组学技术，探讨牛黄解毒片中对雄黄具有配伍减毒作用的主要化学成分群。将24只大鼠随机分成4组：空白组、R组（雄黄组）、RRSPG组（雄黄配伍大黄、黄芩、桔梗和甘草组）、RC组（雄黄配伍大黄总蒽醌、黄芩总黄酮、桔梗总皂苷、甘草总黄酮和甘草总皂苷组），对各组大鼠尿液及血清样品1HNMR谱图进行模式识别分析。结果显示雄黄组大鼠尿液及血清样品代谢轮廓和空白组差异明显，RC组大鼠代谢轮廓同空白组相似，向空白组回归，RC组各化学成分可通过调节能量代谢、胆碱代谢、氨基酸代谢和肠道微生物代谢等方面而起到降低雄黄毒性的作用 [关键词] 代谢组学； 牛黄解毒片； 雄黄； 化学成分群； 配伍减毒 1HNMRbased metabonomics on chemical component groups with toxicity alleviation effect to Realgar in Niuhuang Jiedu tablet XU Wenfeng1， PEI Yuehu2* （1 Department of Pharmacy， Beijing Hospital， Beijing 100730， China； 2 Key Laboratory of TargetBased Drug Design and Research Under Ministry of Education， Shenyang Pharmaceutical University， Shenyang 110016， China） [Abstract] To study the chemical component groups with toxicity alleviation effect to Realgar in Niuhuang Jiedu tablet based on1HNMR metabonomics Twentyfour male Wistar rats were divided into four groups： control group， R group （treated with Realgar）， RRSPG group （treated with Realgar， the root and rhizoma of Rheum palmatum， the root of Scutellaria baicalensis， the root of Platycodon grandiflorum and the root and rhizoma of Glycyrrhiza uralensis） and RC group （treated with total anthraquinones from the root and rhizoma of R. palmatum， total flavonoids from the root of S. baicalensis， total saponins from the root of P. grandiflorum， total flavonoids and saponins from the root and rhizoma of G. uralensis） Based on1HNMR spectra of urine and serum from rats， PLSDA was performed to identify different metabolic profilesThe metabolic profiles of R group were different from that of control group， while the metabolic profiles of RC group were almost similar to control groupTotal anthraquinones from the root and rhizoma of R. palmatum， total flavonoids from the root of S. baicalensis， total saponins from the root of P. grandiflorum， total flavonoids and saponins from the root and rhizoma of G. uralensis regulated energy， choline and amino acid metaboli