葛花枳椇子及其配伍采用水提与醇提对酒精性肝损伤大鼠肝脏功能的影响_柳海艳.pdfVIP

科技导报 ， （ ） 研究论文（ ） 2012 30 28-29 Articles 葛花枳椇子及其配伍采用水提和醇提 对酒精性肝损伤大鼠肝脏功能的影响 柳海艳，钟赣生，王茜，李怡文，刘佳，陈绍红，欧丽娜 北京中 医药大学基础医 学院 ，北京 100029 摘要 为了观察葛花、枳椇子不同比例配伍对酒精性肝损伤大鼠肝脏功能的影响，同时探讨葛花枳椇子及其配伍组采用不同的提 取方法即水提和醇提是否会对药物的疗效产生影响，将Wistar 大鼠，按体重随机分为空白组，模型组，东宝肝泰对照组，葛花组，枳 椇子组，葛花枳椇子配伍 ， 和 （质量比） 组，连续灌胃 周，于 周末取材，麻醉后腹主动脉取血，摘取肝脏，称重计算肝 1:1 1:2 2:1 8 8 8 脏指数，同时检测血清中丙氨酸氨基转移酶（ ）、天门冬氨酸氨基转移酶（ ）、碱性磷酸酶（ ）的含量。 结果显示，造模成 ALT AST ALP 功，各个给药组对于肝功能均有一定的改善作用，同时可以看出水提的效果整体上优于醇提。 由此表明，大量饮酒可以导致肝脏受 到损伤，葛花与枳椇子不同比例配伍对酒精性肝损伤大鼠肝脏具有一定的保护作用，且实验中采用的药物应用传统水提的效果优 于醇提。 关键词 葛花；枳椇子；酒精性肝损伤；肝功能 中图分类号 R285.5 ，R575.5 文献识别码 A doi 10.3981/j.issn.1000-7857.2012.h1.011 Effects of Flos Puerariae and Seeds of Hovenia Dulcis Thunb and Compatibility Groups Extracted by Water and Alcohol on Experimental Alcoholic Liver Disease of Rats LIU Haiyan, ZHONG Gansheng, WANG Xi, LI Yiwen, LIU Jia, CHEN Shaohong, OU Lina College of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China Abstract In order to observe the antioxidant effect of different proportions of flos puerariae lobatae and hoveniae semoveniae semen extracted by alcohol and water, at the same time to determine whether different methods of extraction will have different effects and which method is better, alcoholism rat models were induced by administrating wine and Wistar rats were randomly divided into the normal group, the model group, the positive group, the flos puerariae group, the group of seeds of hovenia dulcis Thunb, and the groups of different ratios of compatibility. All rats were killed and their livers and blood were tak