杨筱慧-2013-向川安瘿蜂和寄生性长尾小蜂共生菌Wolbachia的检测_湖南农业大学学报.pdfVIP

39 3 () Vol.39 No.3 2013 6 Journal of Hunan Agricultural University (Natural Sciences) Jun 2013 DOI:10.3724/SP.J.1238.2013.00274 Wolbachia 1 1* 2 1 (1. 410004 2. 61920) Wolbachia Wolbachia wsp PCR Wolbachia wsp Wolbachia wsp WolbachiaWolbacia Wa Wb wsp Wolbachia Wolbachiawsp Q968 A 1007–1032(2013)03–0274–05 The detection of endosymbiotic bacteria Wolbachia in Andricus mukaigawae and Torymus sp. 1 1* 2 1 YANG Xiao-hui , ZHU Dao-hong , LIU Zhi-wei , SU Cheng-yuan (1.Laboratory of Insect Behavior and Evolutionary Ecology, Central South University of Forestry and Technology, Changsha 410004, China; 2. Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois 61920, USA) Abstract: Wolbachia are maternally inherited endosymbiotic bacteria, and they can manipulate the reproduction of their hosts. In this paper, we amplified the wsp gene fragment of Wolbachia in Andricus mukaigawae and Torymus sp. associated with the plant galls of A. mukaigawae , by using Wolbachia wsp-specific primers. Our results revealed that A. mukaigawae and Torymus sp. were respectively infected with Wolbachia strain W and W . We didn’t found the evidence a b for horizontal transmission between A. mukaigawae and Torymus sp. because of the difference of wsp sequence in Wolbachia strain W and W . a b Key words: Andricus mukaigawae ; Torymus sp.; Wolbachia; wsp gene; horizontal transmission W