四倍体苇状羊茅和多花黑麦草f1 杂种的幼胚培养 - 南京农业大学学报.pdfVIP

( ) 农业大学学报　1997, 20 1 : 9～ 12 　 　 rna l of N anj ing A g ricu ltu ra l U n iversity 四倍体苇状羊茅和多花黑麦草F1 杂种的幼胚培养 及再生植株的形态特征 陈文品　曹明树　张航宁　吴琴生　刘大钧 (南京农业大学细胞遗传研究所, 南京 210095) 摘要　研究了在直接成苗培养基上不萌动的四倍体苇状羊茅和多花黑麦草杂种幼胚通过诱导愈伤组织获 得再生杂种苗的方法。结果表明, 杂种愈伤组织随着继代培养时间的延长, 分化成苗能力逐步下降, 在不改 变培养基的情况下, 分化能力可保持 8 个月; 改变脱分化培养基中N H NO 或 KNO 的浓度可显著提高愈 4 3 3 伤组织的分化能力。此外, 还讨论了再生植株的形态变异。 关键词　四倍体苇状羊茅; 多花黑麦草; 胚培养; 再生苗; 形态特征 中图分类号　 S CUL TURE OF IMM ATURE EM BRYO AND MORPHOLOGICAL CHARACTERS OF ITS REGENERATION PLANT FROM F1 HY BR ID OF TETRAPLO ID TALL FESCUE AND ITAL IAN RY EGRASS Chen W enp in , Cao M ingshu , Zhang H angn ing , W u Q in sheng and L iu D ajun (Cytogenetics In stitu te, N an jing A gric U n iv , N an jing 210095) ABSTRACT　R egeneration p lants w ere obtained from a hybrid imm ature em bryo of tetrap lo id tall fescue ( . . ) ( . ) F estuca arund inacea var g laucascens Bo iss and Italian ryegrass L olium m u ltif lorum L am th rough callus induction and regeneration , w h ich failed to germ inate directly on M S m edium supp lem ented w ith 0. 1 . m g L k inetin T he results show ed that the differentiation ability of em bryo callus decreased w ith the p ro longing of subculture an