应用气相色谱测定化妆品中β-谷甾醇、豆甾醇与菜油甾醇的方法研究.pdfVIP

吾制吾情化妆曰 2012 牢 12 月第6 期 FLAVOUR FRAGRANCE COSMETICS December ,2012 ,NO.6 应用气相色谱测定化妆品中阶谷笛醇、豆笛醇 与菜油笛醇的方法研究头 作费捕食、 1.上海市质量监督检验技术研究院，上海 200233; 强￡辉0971:-)，女，本科，南极;品程师， 2. 上海市质量技术监督局，上海 200233; 主接从事食品J总精品栓糊不作。 联革电话:021\是 3. 华东师范大学生命科学学院，上海 200241 张辉1 曹程明2 吕 庆1 程宗德3 麦成华1 建立了以气相色谱定量测定化妆品中什谷面醇、豆面醇和菜油画醇 3 种植物简醇含量的方法。化妆品样品以 5-c，-胆面:院 为内标，通过氢氧化饵-乙醇溶液皂化、正己烧提取、硅皖化衍生后，采用毛细管气相色谱柱分离，氢火焰离子化检测器(FID) 定量检测。 3 种植物1ií醇的线性相关系数 (R )均0.)))0 ，以信噪比 S/N= 3 计算的白谷面醇、豆筒醇和菜油画醇最低检出 浓度分别为1. 06 mg/LJJ. 86 mg/L 和 0.78 mg/L ，样品加标回收率在 76%~118%之间，相对标准偏差为 O.8%~6.1% 。 e噩噩 植物筒醇类自谷面醇豆面醇菜油画醇化妆品气相色谱法 Study On the Determination of P-Sitosterol , Stigmasterol and Campesterol in Cosmetics by Gas Chromatog- raphy with Flame Ionization Detection 祷 ZHANGHui 1 CAOChengming 2 LVQing 1 ZHAI Zongde 3 MAIChenghua 1 (1. Shanghai Institute of Quality Inspection and Technical Research , Shanghai 200233 , China; 2. Shanghai Municipal Bureau of Quality and Technical Supervisio口， Shanghai 200233 , China; 3. Schoolof Life Science , East China Normal University , Shanghai 200241 , China) Abstract: A method for simultaneous determination of ß-sitosterol , stigmasterol and campesterol in cosmetics was established using capillary gas chromatography. The samples , with 5αcholestane added as an internal standard , were saponified with po tassium hydroxide in ethanolic solutio口， followed by hexane extractio口 and trimethylsilyl ether derivatization , and were finally detected by FID. The correlation coefficients 口f three kinds of phytosterols were all over O. )))0 , th