建立检测祛痘类化妆品中违禁添加维A酸的液相分析法.pdfVIP

580 中国药事 2014 年第 28 卷第 6 期 建立检测桂症类化妆晶中违禁添加维 A 酸的液相分 析法 严晓明，谭妙娟1 ，何炳洪2 (广东省韶关市食品药品检验所，韶关 512026; l 广东省食品药品 职业技术学校; 2 南方医科大学第三附属医院) 摘要: 目的建立检测手去症类化妆品中非法添加违禁成分维 A 酸的方法，为化妆品的监管提供技术支撑。 方法色谱柱采用 Agilent Poroshell 120 EC-C18 (3. OmmX 100mm , 2.7μm) ，甲醇- 0.5% 冰醋酸 (90: 10) 为流动相，检测波长 350nm ，流速1. OmL • min-1 1. 012~20. 24μg 范围内与 A 酸进样量在 。结果维 峰面积呈良好线性关系，相关系数 r=O. 9997 ，加样回收率为 99.8% ， RSD=0.4% (n=9) ，检测限 (SI N=3) 为 0.002μg. mL- 1 0 结论本文建立的液相分析法经方法学验证可用于化妆品违禁添加维 A 酸的快 速检测。 关键词: 化妆品;维 A 酸;维甲酸;非法添加;液相分析法 中图分类号: R284. 1; R286. 0 文献标识码:A 文章编号: 1002-7777 (2014) 06-0580-04 Establishment of a Method for Liquid Chromatographic Analysis for Determination of Tretinoin Illegally Added in Acne-removing Cosmetics Yan Xiaoming , Tan Miaojuan 1 and He BinghongZ (Shaoguan Institute for Drug and Food Control , Shaoguan 512026; 1 Guangdong Food and Drug Vocational- Technical School; Z The Third Affiliated Hospital of Southern Medical University) ABSTRACT: Objective To establish a fast liquid chromatographic method for determination of illegally added tretinoin in acne-removing cosmetics and provide technical support for supervision for cosmetics. Methods The chromatographic column was Agilent Poroshell 120 EC-C18 (3. Omm X 100 mm , 2.7μm); the mobile phase consisted of methanol-O. 5% glacial acetic acid (90: 10); the detection wavelength was 1 350 nm; the flow rate was 1. 0 mL • min- • Results The linear range of tretinoin was 1. 012 - 20. 24μg with r=0.9997. The average recover