人工蛹虫草的化学成分分析.PDFVIP

36 2013, Vol.34, No.11 食品科学 ※基础研究 人工蛹虫草的化学成分分析 * 陈 策，图力古尔 ，包海鹰 (吉林农业大学 食药用菌教育部工程研究中心，吉林 长春 130 118) 摘  要：采用硅胶柱色谱法、薄层色谱法、紫外-可见吸收光谱分析法、核磁共振法、液质联用(HPLC-MS/MS) 的多反应监测(MRM)等多种分离与鉴定方法，从人工培养蛹虫草的石油醚、氯仿和甲醇层提取物中分离鉴定出 8个化合物，并通过GC-MS技术对人工蛹虫草石油醚提取物进行分析，得出其脂肪酸以亚油酸和反油酸为主， 含量分别为4 1.949% 、27 .696% 。分离得到的8个化合物结构分别被鉴定为：玉米黄素、二氢胆甾醇油酸酯、1- β 油酰基-2-亚油酸-3-棕榈酸甘油、4 ,6,8( 14),22(23)- 四烯-3-酮-麦角甾烷、 -谷甾醇、麦角甾醇过氧化物、啤酒 甾醇、甘露醇，其中玉米黄素、二氢胆甾醇油酸酯、1- 油酰基-2-亚油酸-3-棕榈酸甘油均是首次从蛹虫草中分 离得到。 关键词：玉米黄素；二氢胆甾醇油酸酯；1-油酰基-2-亚油酸-3-棕榈酸甘油；蛹虫草 Chemical Composition Analysis of Cultured Cordyceps militaris * CHEN Ce ，BAU Tolgor ，BAO Hai-ying (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130 118, China) Abstract ：Eight compounds were isolated from cultured Cordyceps militaris by silica gel column chromatography and recrystallization. Their structures were identified as zeaxanthin, β -cholestanyl oleate, 1-oleoyl-2-linoleoyl-3-palmitoylglycerol, 4,6,8( 14),22(23)-tetraen-3-one-ergostane, β -sitosterol, ergosterol peroxide, cerevisterol and mannitol by thin layer chromatography (TLC), HPLC-MS/MS, NMR and EI-MS. This study is the first report that zeaxanthin, β -cholestanyl oleate, and 1-oleoyl-2-linoleoyl-3-palmitoyl glycerol were isolated from Cordyceps militaris. linoleic acid The maj or fatty acids of petroleum ether extract of cultured Cordyceps militaris were linoleic acid and elaidic acid, acounting for 4 1.949%