pAc5.1V5-His B昆虫表达载体说明.pdfVIP

pAc5.1/V5-His B 编号 载体名称 北京华越洋生物KCVECT119 pAc5.1/V5-His B pAc5.1/V5-His B 载体基本信息 载体名称: pAc5.1-V5-His B 、pAc5.1/V5-His B 质粒类型: 昆虫表达载体；果蝇细胞表达载体 克隆方法: 多克隆位点，限制性内切酶 启动子: P-AC5 (Actin 5C) 载体大小: 5379 bp 5 测序引物及序列: AC5 Forward ：5d[ACACAAAGCCGCTCCATCAG]3 3 测序引物及序列: -- 载体标签: V5 Epitope Tag , His Tag (C-末端) 载体抗性: 氨苄青霉素 真核筛选标记: 无 克隆菌株: TOP10 ；DH5-T1R 宿主细胞（系）: 果蝇细胞系S2 pAc5.1-V5-His 载体可以与筛选载体pCoHygro 或者pCoBlast 共转染， 备注: 建立稳表达细胞系。 瞬表达/稳表达: 瞬表达 组成型/诱导型: 组成型 病毒/非病毒: 非病毒 pAc5.1/V5-His B 载体质粒图谱和多克隆位点信息 pAc5.1/V5-His B 载体简介 The DES-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features: Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag. pAc5.1/V5-His B 载体序列 ORIGIN 1 CTTCAAGGAA TTAATTCTAT ATTCTAAAAA CACAAATGAT ACTTCTAAAA AAAATCATGA 61 ATGGCATCAA CTCTGAATCA AATCTTTGCA GATGCACCTA CTTCTCATTT CCACTGTCAC 121 ATCATTTTTC CAGATCTCGC TGCCTGTTAT GTGGC