白灵菇液体培养工艺研究.pdfVIP

安徽农业科学，Journal ofAnhui Agri．Sci．2007，35(35)：11382—11383 责任编辑 姜 丽 责任校对 王 淼 白灵菇液体培养工艺研究 黄聪亮1t唐玉琴2(1．漳州职业技术学院食品与生物工程系，福建漳州3630o0；2．吉林农业科技学院生物工程系，吉林吉林132101) 摘要 『目的1为白灵菇进一步开发提供科学依据。『方法】采用单因素试验和正交试验，确定白灵菇液体培养的最适碳源、氮源和最佳 培养条件。『结果1适合白灵菇液体培养的碳源是玉米粉和葡萄糖，氮源是黄豆粉和蛋白胨。碳源和氮源的主次因素为黄豆粉葡萄糖 玉米粉蛋白胨。最佳碳、氮源组合为玉米粉3．0％，葡萄糖1．5％，黄豆粉2．0％，蛋白胨0．2％，生物量为1．781 l0o ml。在培养条件各 因素中，主次因素为pH值接种量转速装液量。pH值对茵丝生物量有极其显著的影响，接种量、转速、装液量的影响不明显。[HOe1 最佳培养条件是摇瓶装液量80ml／250ml，接种量10％，pH值6．5，摇瓶转速180 r／min，25 oC培养8 d，生物量为1．792 g／10oml。 关键词 白灵菇；液体培养；正交试验 中图分类号 $646 文献标识码 A 文章编号 o517—6611(2007)35—11382—02 Study on Liquid Culture Technology for ofP urotus nebrodens~ HUANG Cong-liang et al(Department of Food and Bioengineering，Zhangzhou Vocational Technical College，Zhangzhou，Fujian 363000) Abstract 『Objective1 The research aimed to provide scientific basis for further development of Heurotus nebrodensis．『Method1 The optimum carbon source，nitrogen source and the best culture conditions were confirmed through single factor test and orthogonal test．『Result1 The suitable carbon source for liquid culture of P nebrodensis was corn meal and glucose．the suitable nitrogen source was bean flour and peptone．The factors of carbon source and nitrogen were in sequence as：bean flourglucosecolTl mealpeptone．The optimum combination of carbon source and nitrogen source was 3％corn meal，1．5％glucose，2％bean flour and 0．2％peptone，with the biomass of 1．78 1 g／100 m1．The culture conditions were in sequence as：pH valueinoculation amountrotational speedliquid filling amount．pH value had an extremely significant effects on the mycelial biomass，while inoculation amount，rotational speed and liquid filling amount had not obvious effect．『Conclusion1 The optimum culture conditions were as follows：Liquid filling amount per shake flask of 80 ml／250 m1．inoculation amount of 10％，pH value of 6．5．rotational speed ofshake flake of 180 r／min．culturing 8 days at