粉防己碱脂质体药物含量及包封率的测定.pdfVIP

第26卷第4期 河南火学学报(医学版) VoI．26 No．4 2007年 l 1月 Journal of Hemm University(Medical Science) NOV．2007 粉防己碱脂质体药物含量及包封率的测定 陈洪轩，王艳丽 (河南大学药学院药剂教研室，河南-矸封475004) 摘 要：目的：建立Tet脂质体药物含量测定及包封率测定的反相高效液相色谱法、方法：反相高效液相色谱法。 采用Kromasil CI8(250 mnl×4．6 mill，5 Ixm)；流动相：甲醇一乙腈 水一0．8％冰醋酸(30：15：55)；选样量：20 Ixl；流速： 1．0 ml／min；紫外检测波长：280 nm。采用葡聚糖凝胶G一50柱色谱法分离Tet脂质体和游离药物。结果：在本色 谱条件下Tet与辅料及溶剂峰分离良好，Tet在2．0 n1g／L～100．0 mg／L范围内线性关系良好(r：0．999 9，17,=3)，平 均回收率在lO0 O％～l01．0％之间。目内精密度RSD及日间精密度RSD均小于2％ (n：5)。结论：该方法准确 可靠、简便易行，可用于Tet脂质体药物含量及包封率的测定 、 关键词：粉防己碱；脂质体；含量测定；包封率 中图分类号：R972．3 文献标识码：A 文章编号：l672—7606(2007)04—0038—03 Determination of Tetrandrine liposome medicine contents and its entrapment efficiency CHEN Hong—xuan，WANG Yan—li (Medicament Department Pharmaceutical College，Henan University，Henan Ka ng 475004，China) Abstract：0 ective： To establish a RP—HPI C method tbr the contents arid the entrapment efficiency determination of Tet lipnsome．Methods： Reversed phase high-performanc．e liquid chromatography was used with chromatographic column：Kromasil Cl8(250 mm 4．6 mm，5 m)；mobile phase：methylalcohol—second grade nitrile—water一0．8％ glacial acetic acid(30：l 5：55)；sample size：20 l；Flow rate：1．0 mlj min ；ultravinlet examination wavelength：280nm．The polydextran gel G-50 column chromatography was set up tn separate the Tet liposome and free medicine．Results： Under this ehromatogTaph condition，Tet was separated well from the supplementary raaterial and the solvent peak had a good linear relation in the range of 2．0 mg／1 ～l00．0 mg／L(r：0．9999，n=3)．Average 1 ecovery is between 100．0％ 一101． 0％． [he intra4tay RSD aml intm lay RSD were leSS than 2％(n=5)．Conclusion： Fhis method is simple，accurate， sensitive an~l applica1)le findetermination of the contents and entrapment effic