混合共轭聚合物纳米粒子介导的荧光能量转移.ppt

Changfeng Wu, Hongshang Peng,? Yunfei Jiang, and Jason McNeill* Energy Transfer Mediated Fluorescence from Blended Conjugated Polymer Nanoparticles Abstract Nanoparticles consisting of a derivative of the blue-emitting conjugated polymer polyfluorene doped with green-, yellow-, and red-emitting conjugated polymers were prepared by a reprecipitation method. The nanoparticles can be described as a system of densely packed chromophores that exhibit efficient energy transfer from the host to the dopant polymers. Abstract Fluorescence quenching analysis of the host polymer as a function of the dopant concentration indicates that one energy acceptor molecule can effectively quench 90% of the fluorescence of a nanoparticle consisting of 100-200 host conjugated polymer molecules. A nanoparticle energy transfer model was developed that successfully describes the quenching behavior of a small number of highly efficient energy acceptors per nanoparticle. Abstract The fluorescence brightness of the blended polymer nanoparticles was determined to be much higher than that of inorganic quantum dots and dye-loaded silica particles of similar dimensions. The combination of high fluorescence brightness and tunable fluorescence of these blended nanoparticles is promising for ultrasensitive fluorescence-based assays. Contents 1. Introduction 2. Experimental Section 3. Results and Discussion 4. Conclusions 1.Introduction Highly fluorescent nanoparticles have attracted much attention due to a variety of fluorescence-based applications such as biosensing, imaging, and high-through put assays. Conjugated polymers are known to possess high absorption coefficients and high fluorescence efficiency, which have led to a wide range of applications in optoelectronic thin film devices. However, the use of conjugated polymer nanoparticles in fluorescence labeling is still a largely unexplored area. 1.Introduction Energy transfe