苏丹红I完全抗原设计及制备.pdfVIP

中国卫生检验杂志2007年l2月第l7卷第l2期 Chinese Journal ofHealth Laboratory Technology，Dec 2007；Vol 17 No 12 2131 【论著】 苏丹红I完全抗原设计及制备 任立松 ，党荣理 ，杨林 ，马永红 ，王颖 ，邱凯 ，吴静怡 ，柳增善 ’ (1．吉林大学畜牧兽医学院，长春 130062； 2．吉林大学人兽共患病研究所，长春 130062；3．新疆军区疾病预防控制中心，乌鲁木齐 830011) [摘要] 目的：制备苏丹红I完全抗原。方法：根据苏丹红I(Sudan—I)分子特点，合成Sudan—I结构类似物，与su— dan—I结构相比，该结构类似物在其偶氮环对位引进一个羧基。通过碳二亚胺法(EDC)与牛血清蛋白(BSA)载体蛋白 偶联，偶联产物经透析纯化后，用SDS凝胶电泳、紫外扫描鉴定，Sudan—I结构类似物与BSA的平均偶联比达到2～3 个。用该偶联物免疫小鼠，获得抗苏丹红I血清。结果：成功制备了苏丹红I完全抗原。结论：通过合成结构类似物，与 大分子物质偶联，获取抗苏丹红I血清是可行的。 [关键词] 苏丹红I；结构类似物；抗原 [中图分类号】 R155．5 [文献标识码】 A [文章编号】 1004—8685(2007)12—2131—03 Synthesis of complete antigen of Sudan——I and its antibody production Ren Li．song r，Dang Rong-li。Yang Lin。Ma Yong。ho ，Wang Ying‘，Qiu Kai‘，Wu．1ing～yi，Liu Zeng—shan “ ， ， (1．College ofAnimal Science and Veterinary Medicine，Jilin Univercity，Changchun 130062，China；2，Institute ofZoonosis，Jilin University，Changchun 130062，China；3．Xinjiang Military Center for Disease Control and Prevention，Urumqi 830011，China) [Abstract] Objective：To synthesize complete antigen of Sudan—I．Methods：Based on the molecular structure of Sudan— I。a analogy of Sudan —I Was synthesized．Compared to the molecular structure of Sudan—I，a carboxyl group Was induced to the counterpoint of chemical group—N=N．then coupled with BSA through EDC to be complete antigen．The completed antigen Was dialyzed and identified by SDS—PAGE。UV—scanning．The coupling rate in average Was 2～3．The blood serum of anti—Su- dan—1 were obtained by immunizing the mice．Results：The complete antigen of Sudan—I Was obtained．Conclusion：Synthe- sized the analogy ofthe structure．and coupled with molecule．it Was feasible to obtain the serum of anti—Sudan～I． [K