西班牙南部中心地区白粉病种群对DMI类杀菌剂的敏感性.pdfVIP

Research Article Received: 22 October 2009 Revised: 15 January 2010 Accepted: 2 February 2010 Published online in Wiley Interscience: 29 March 2010 () DOI 10.1002/ps.1948 Sensitivities to DMI fungicides in populations of Podosphaera fusca in south central Spain ´ a ´ b∗ Francisco J Lopez-Ruiz, Alejandro Perez-García, ´ ˜ a b c Dolores Fernandez-Ortuno, Diego Romero, Emilio García, b d ´ a Antonio de Vicente, James KM Brown and Juan A Tores Abstract BACKGROUND: Cucurbit powdery mildew elicited by Podosphaera fusca (Fr.) U Braun N Shishkoff limits crop production in Spain. Disease control is largely dependent on fungicides such as sterol demethylation inhibitors (DMIs). Fungicide resistance is an increasing problem in this pathogen. To overcome such risk, it is necessary to design rational control programmes based upon knowledge of field resistance. The aim of this study was to investigate the state of DMI sensitivity of Spanish P. fusca populations and provide tools for improved disease management. RESULTS: Using a leaf-disc assay, sensitivity to fenarimol, myclobutanil and triadimenol of 50 isolates of P. fusca was analysed to determine discriminatory concentrations between sensitive and resistant isolates. As no clearly different groups of isolates could be identified, discriminatory concentrations were established on the basis of maximum fungicide field application rate, 100 mg L−1 for the three fungicides tested. Subsequently, a survey of DMI resistance was carried out in dif