稀土元素金属螯合标记结合QTOFMS的定量蛋白质组新方法论文.pdf

V01．27 第27卷增刊 质谱学报 Suppl． 2006年7月 ofChineseMass Jul．2006 Journal SpectrometrySociety 稀土元素金属螯合标记结合Q-TOF-MS的定量蛋白质组新方法 2 刘慧玲1’2’3张养军h2，钱小红1’ (1．北京放射医学研究所，北京 100850；2．北京蛋白质组研究中心，北京100850； 3．jE京输血医学研究所，北京100850) for ANewMethod ProteomicsResearch Quantitative Element Metal Chelatedand byUsing Tags Q。·TOF--MS LIU Hui—lin91·2一，ZHANGYang-junl“，QIANXiao-hon91·2 Institute Medicine，27 100850，China； (1．BeoingofRadiation TaipingRoad，Beijing ProteomeResearchCenter，33 2．Beijing ZhongguancunLi知ParkRoad，Beijing102206，China； Institute Medicine，27 100850，China) 3．Beijing ofTransfusion TaipingRoad，Beijing Abstract：Theuseofsomestable haslimitationsduetorelative and isotoperegents highprice anew difficulties；asaresult，we methodfor re- synthesis developed quantitativeproteomics metalelement withmass search chelated byusing tags(MECT)coupledspectrometry．The ofthemethodliesthatthe anhydride principle bicyclic is tothe aminesof thenchelatedtorare pentaaceticcovalently primary peptides，and coupled metalYandTb．After modifications，the aremixedand peptide taggedp