合成高级醇的微生物细胞工厂研究进展.pdfVIP

刘增然 等/合成高级醇的微生物细胞工厂研究进展 1421 生 物 工 程 学 报 Chinese Journal of Biotechnology October 25, 2013, 29(10): 1421−1430 /cjbcn ©2013 Chin J Biotech, All rights reserved 综 述 合成高级醇的微生物细胞工厂研究进展 刘增然, 张光一 河北经贸大学生物科学与工程学院，河北 石家庄 050061 刘增然, 张光一. 合成高级醇的微生物细胞工厂研究进展. 生物工程学报, 2013, 29(10): 1421–1430. Liu ZR, Zhang GY. Advance in producing higher alcohols by microbial cell factories. Chin J Biotech, 2013, 29(10): 1421–1430. 摘 要: 高级醇疏水性好，能量密度高，能与汽油随意混合；用微生物发酵可再生材料生产高级醇燃料替代矿 物燃料是发展趋势。文中综述了构建合成高级醇的酿酒酵母和大肠杆菌细胞工厂的研究和相关技术平台。重点 介绍了依赖CoA 的梭菌途径和α-酮酸介导的非发酵途径的构建，分析了各自的特点，总结了生产高级醇的微 生物细胞工厂的构建策略；提出高级醇工业化生产要解决的问题和研究方向。 关键词: 高级醇，α-酮酸，氨基酸代谢，系统代谢工程 Advance in producing higher alcohols by microbial cell factories Zengran Liu, and Guangyi Zhang College of Bioscience and Bioengineering, Hebei University of Economics and Business, Shijiazhuang 050061, Hebei, China Abstract: Higher alcohols have a high energy density, low hygroscopicity and can be mixed with gasoline at any ratio. It is the trend to replace fossil fuels with biofuels produced via microbial fermentation of renewable resources. We reviewed the progress in the development of engineered Saccharomyces cerevisiae and Escherichia coli that can produce higher Received: June 4, 2013; Accepted: August 19, 2013 Supported by: Technology Foundation for Returned Overseas Chinese Scholars, Ministry of Human Resources and Social Security of Hebei (No. 2011-226), Science Research Foundation of the Higher Education Institutions of Hebei Province (No. ZH2012056), Program of Key Developing Discipline of Hebei University of Economics and Business. Corresponding author: Zengran Li