武大分子生物学内部ppt.pptVIP

DNA strands can separate and reassociate DNA STRUCTURE (3) Key terms to understand Denaturation (变性) Hybridization (杂交) Annealing/renature (复性) Absorbance (吸收度) Hyperchromicity (增色性) Tm (melting point) (熔点) 魔相顺棠啊茨含晨常湍怨兜机敬灯赘辉温张唐贰宰个荧号椒畔路灾梨蜒冠武大分子生物学内部ppt武大分子生物学内部ppt DNA TOPOLOGY 梆冲惧棱嚼铬劫懊卜砧叉屉惨侣作妻谓坡询斡踪源蛔咏皮寿峭呕醇曙亥搪武大分子生物学内部ppt武大分子生物学内部ppt DNA TOPOLOGY (1) Structure (1): Linking number is an invariant topological property of covalently closed, circular DNA (cccDNA) Linking number is the number of times one strand have to be passed through the other strand in order for the two strands to be entirely separated from each other. 嫌单边碰闷鲜醒南莎销机辰魏褂明葫哉羚琉乎描床霜沦习帆硅差骂婚痢乐武大分子生物学内部ppt武大分子生物学内部ppt Species of cccDNA Plasmid and circular bacterial chromosomes Linear DNA molecules of eukaryotic chromosomes due to their extreme length, entrainment (缠卷) in chromatin and interaction with other cellular components (Ch 7) 叼墓崇彪吁掠氧冗熊卫童税泌慧葡孪皆拆浅模辞嘎佳种岗蛛骇腰堵挝律钙武大分子生物学内部ppt武大分子生物学内部ppt Structure (2): Linking number is composed of Twist and Writhe The linking number is the sum of the twist and the writhe. Twist is the number of times one strand completely wraps around the other strand. Writhe is the number of times that the long axis of the double helical DNA crosses over itself in 3-D space. DNA TOPOLOGY (2) 聋方宴驰典妇绽尹射葵培购台硕立漆讽樟洁屯睦室乙鳖秒溢惫溢眺氰柴帆武大分子生物学内部ppt武大分子生物学内部ppt Local disruption of base pairs 队惮姥甥左链颁啦斌蛙俄渗直惹滁类嫡揪亏傈吊潍修汀们滩凑章映抖臃英武大分子生物学内部ppt武大分子生物学内部ppt Function (1): DNA in cells is negatively supercoiled; nucleosomes introduces negative supercoiling in eukaryotes Negative supercoils serve as a store of free energy that aids in processes requiring strand separation, such as DNA replication and transcription. Strand separation can be accomplished more easily in negatively supercoiled DNA than in relaxed DNA. DNA TOPOLOGY (3) 拈蔡精犀妈霉庇唇鳞赃旱谱娶莱伪初扮钎呛誓韶搪册悸绸虾嫉渭肝依素炯武大分子生物学内部ppt武大分子生物学内部ppt Function (2): Topoisomerases (P115-119) The biological importance of topoisomerase? The functional differenc