Skp2和PTEN在人脑星形细胞瘤中的表达及临床意义.pdfVIP

生垦塞旦 经 瘟盘查 8年1月第11卷第1期 Chinese Journal of Practical Nervous Diseases Jan．2008，Vo1．1I No．1 · 9 · Skp2和PTEN在人脑星形细胞瘤中的表达及临床意义 郑 杰 宋来君 孙红卫 杜 伟 党 博 郑州大学第一附属医院神经外科 郑州 450052 【摘要】 目的 探讨Skp2和PTEN在人脑星形细胞瘤中的表达及与病理分级的关系。方法 免疫组化S-P法检测7O例I ～ Ⅳ级脑星形细胞瘤和8例正常脑组织标本中Skp2和PTEN的表达。结果 (1)Skp2、PTEN在人脑星形细胞瘤组中阳性表达率 分别为47．1％和50．0％，而在正常脑组织组中阳性表达率分别为0％和100％。从星形细胞瘤I、II级组，III级组到IV级组，Skp2 的阳性表达率呈增高趋势，分别为9．1 、45．5％、80．8％，而PTEN的阳性表达率呈降低趋势，分别为90．9％、50．0％、15．4 。 (2)Spearman等级相关检验证实病理分级和Skp2标记指数呈正相关，和PTEN标记指数呈负相关。人脑星形细胞瘤中Skp2和 PTEN的表达呈负相关。结论 Skp2和PTEN蛋白的表达情况可作为人脑星形细胞瘤的诊断和恶性程度评估的参考资料，可能 成为人脑星形细胞瘤治疗的新靶点。 【关键词】 星形细胞瘤；Skp2；PTEN；免疫组织化学 【中图分类号】 R730．264 【文献标识码】 A 【文章编号】 1673—5110(2008)01—0009—03 Expression and clinical significance of Skp2 and PTEN in astrocytoma Zheng Jie，Song Laijun，Sun Hongwei，et a1． Department of Neurosurgery。the First A liated Hospital to Zhengzhou University。Zhengzhou 450052 [Abstract]Objective To investigate the expression of Skp2 and PTEN in the astrocytoma，the relationship between the tow and pathological classification．Methods The expressions of Skp2 and PTEN were detected by using immunohistochemical SP staining in 70 cases of astrocytoma(grade I to IV)and 8 cases of normal brain tissues．Results (1)The positive rate of the expression of Skp2 and PTEN in astrocytoma were 47．1 and 50．0 。and those in normal brain tissues were 0 and 100 respectively．From astrocytoma of grade I，II，grade III to grade IV，the positive expression of Skp2 raised gradually，the positive rates were 9．1％， 45．5 and 80．8 。respectively．And PTEN positive expression decreased gradually，the positive rates were 90．9 ，50．0 and 1 5．4％，respectively．(2)Using Spearman rank correlation test，it could be confirmed that pathologic classification was positively cor— related with Skp2 expression index and negatively correlated with PTEN expression index．Skp2 expression was negatively correlated with PTEN expression in astrocytoma．Conclusion The expression of S